Back to Search
Start Over
Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways
- Source :
- Oncology Reports
- Publication Year :
- 2015
- Publisher :
- Spandidos Publications, 2015.
-
Abstract
- Phloretin (Ph) existing in apples, pears and various vegetables is known to have antitumor activities in several cancer cell lines. However, little is known about its effect on human lung cancer cells. The aim of the present study was to see whether Ph could induce apoptosis of non-small cell lung cancer (NSCLC) cells, and explore the possible underlying mechanism of action. We found that Ph markedly induced cell apoptosis of NSCLC cell line A549, and inhibited the migration of A549 cells in a dose-dependent manner. The expression level of BAX, cleaved caspase-3 and -9, and degraded form of PARP was increased and Bcl-2 was decreased after Ph treatment. In addition, the phosphorylation of P38 MAPK, ERK1/2 and JNK1/2 was increased in a dose‑dependent manner in parallel with Ph treatment. Inhibition of P38 MAPK and JNK1/2 by specific inhibitors significantly abolished the Ph-induced activation of the caspase-3 and -9. In vivo tumor-suppression assay further indicated that Ph (20 mg/kg) displayed a more significant inhibitory effect on A549 xenografts in tumor growth. All these findings indicate that Ph is able to inhibit NSCLC A549 cell growth by inducing apoptosis through P38 MAPK and JNK1/2 pathways, and therefore may prove to be an adjuvant to the treatment of NSCLC.
- Subjects :
- P38 MAPK
Cancer Research
MAP Kinase Signaling System
Phloretin
p38 mitogen-activated protein kinases
Cell
Biology
migration
p38 Mitogen-Activated Protein Kinases
Mice
chemistry.chemical_compound
Cell Movement
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
medicine
Animals
Humans
Mitogen-Activated Protein Kinase 9
Mitogen-Activated Protein Kinase 8
Phosphorylation
Cell Proliferation
A549 cell
Oncogene
Cell growth
apoptosis
Articles
General Medicine
Cell cycle
Xenograft Model Antitumor Assays
respiratory tract diseases
Gene Expression Regulation, Neoplastic
lung cancer
medicine.anatomical_structure
Oncology
chemistry
Apoptosis
Cancer research
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....fa8eb6a07c6065fd94bb93db9ee5481c