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Gypenosides protects dopaminergic neurons in primary culture against MPP(+)-induced oxidative injury
- Source :
- Brain research bulletin. 83(5)
- Publication Year :
- 2010
-
Abstract
- Oxidative injury has been implicated in the etiology of Parkinson's disease (PD). Gypenosides (GPs), the saponins extract derived from the Gynostemma pentaphyllum, has various bioactivities. In this study, GPs was investigated for its neuroprotective effects on the 1-methyl-4-phenylpyridinium ion (MPP(+))-induced oxidative injury of dopaminergic neurons in primary nigral culture. It was found that GPs pretreatment, cotreatment or posttreatment significantly and dose-dependently attenuated MPP(+)-induced oxidative damage, reduction of dopamine uptake, loss of tyrosine hydrolase (TH)-immunopositive neurons and degeneration of TH-immunopositive neurites. However, the preventive effect of GPs was more potential than its therapeutical effect. Most importantly, the neuroprotective effect of GPs may be attributed to GPs-induced strengthened antioxidation as manifested by significantly increased glutathione content and enhanced activity of glutathione peroxidase, catalyze and superoxide dismutase in nigral culture. The neuroprotective effects of GPs are specific for dopaminergic neurons and it may have therapeutic potential in the treatment of PD.
- Subjects :
- 1-Methyl-4-phenylpyridinium
Dopamine
education
Substantia nigra
Pharmacology
medicine.disease_cause
Neuroprotection
Superoxide dismutase
chemistry.chemical_compound
Pregnancy
medicine
Animals
Rats, Wistar
Cells, Cultured
chemistry.chemical_classification
Neurons
biology
Dose-Response Relationship, Drug
Chemistry
Herbicides
Plant Extracts
General Neuroscience
Glutathione peroxidase
Dopaminergic
Neurotoxicity
Glutathione
medicine.disease
Gynostemma
Rats
Substantia Nigra
Oxidative Stress
Neuroprotective Agents
Biochemistry
biology.protein
Female
Reactive Oxygen Species
Oxidative stress
Subjects
Details
- ISSN :
- 18732747
- Volume :
- 83
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Brain research bulletin
- Accession number :
- edsair.doi.dedup.....fa7bc16b4b795b22cb9985553bd60ed9