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A proteomic investigation of soluble olfactory proteins in Anopheles gambiae
- Source :
- PLoS ONE, Vol 8, Iss 11, p e75162 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector.
- Subjects :
- Genetics and Molecular Biology (all)
Arthropod Antennae
Male
Proteomics
crystal-structure
regenerating legs
odorant-binding-protein
malaria-transmitting mosquito
repellent deet
chemosensory proteins
mechanism
ligand-binding
bombyx-mori
pheromone-sensitive neurons
media_common.quotation_subject
Anopheles gambiae
Protein domain
lcsh:Medicine
Insect
Receptors, Odorant
Bioinformatics
Biochemistry
Genome
insect chemical communication
Receptors
Anopheles
Animals
lcsh:Science
Gene
media_common
Female
Gene Expression Profiling
Insect Proteins
Sex Characteristics
Agricultural and Biological Sciences (all)
Biochemistry, Genetics and Molecular Biology (all)
Medicine (all)
Multidisciplinary
biology
fungi
lcsh:R
biology.organism_classification
Gene expression profiling
Odorant
Cardiovascular and Metabolic Diseases
lcsh:Q
Technology Platforms
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....fa6d53c70af823a3af64ff17e91c585d