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SDF-1 preconditioned HPC scaffolds mobilize cartilage-derived progenitors and stimulate meniscal fibrocartilage repair in human explant tissue culture
- Source :
- Connect Tissue Res
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- PURPOSE: The purpose of this study was to characterize the influence of SDF-1 on cell migration/adhesion and temporal gene expression of human cartilage mesenchymal progenitor cells (C-PCs); and to utilize SDF-1 conditioned mesenchymal progenitors to stimulate reintegration of human meniscus fibrocartilage breaks. MATERIALS AND METHODS: Characterization of SDF-1-induced cell migration was achieved using hydroxypropyl cellulose (HPC) scaffolds pretreated with SDF-1. Fluorescence microscopy and cell counting were used to visualize and quantify the extent of cell migration into scaffolds, respectively. Relative mRNA expression analysis was used to characterize the temporal effects of SDF-1 on C-PCs. Tissue reintegration experiments were conducted using cylindrical human meniscal tissue punches, which were then placed back together with an HPC scaffold embedded with C-PCs. Tensile testing was used to evaluate the extent of tissue reintegration stimulated by human mesenchymal progenitors. RESULTS: C-PCs migrate into scaffolds in response to SDF-1 with the same efficiency as mesenchymal progenitors from human marrow (BM-MSCs). SDF-1 treatment of C-PCs did not significantly alter the expression of early and late stage chondrogenic differentiation genes. Scaffolds containing SDF-1 preconditioned C-PCs successfully adhered to fibrocartilage breaks and migrated from the scaffold into the tissue. Tensile testing demonstrated that SDF-1 preconditioned C-PCs stimulate reintegration of fibrocartilage tears. CONCLUSION: C-PCs migrate in response to SDF-1. Exposure to SDF-1 does not significantly alter the unique mRNA profile of C-PCs that make them desirable for cartilaginous tissue repair applications. SDF-1 pretreated mesenchymal progenitors successfully disperse into injured tissues to help facilitate tissue reintegration.
- Subjects :
- 0206 medical engineering
02 engineering and technology
Menisci, Tibial
Biochemistry
Article
Tissue Culture Techniques
03 medical and health sciences
Tissue culture
Rheumatology
medicine
Cartilaginous Tissue
Humans
Orthopedics and Sports Medicine
Progenitor cell
Cellulose
Molecular Biology
030304 developmental biology
0303 health sciences
Tissue Scaffolds
Chemistry
Cartilage
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell migration
Cell Biology
Chondrogenesis
020601 biomedical engineering
Chemokine CXCL12
Tibial Meniscus Injuries
Cell biology
medicine.anatomical_structure
Fibrocartilage
Subjects
Details
- ISSN :
- 16078438 and 03008207
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Connective Tissue Research
- Accession number :
- edsair.doi.dedup.....fa6833571817256136c8ab55104b4c4e
- Full Text :
- https://doi.org/10.1080/03008207.2019.1689966