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Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination
- Source :
- ACS Sustainable Chemistry and Engineering, 8(25), 9301-9310, Blossom, B M, Russo, D A, Singh, R K, Van Oort, B, Keller, M B, Simonsen, T I, Perzon, A, Gamon, L F, Davies, M J, Cannella, D, Croce, R, Jensen, P E, Bjerrum, M J & Felby, C 2020, ' Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination ', ACS Sustainable Chemistry and Engineering, vol. 8, no. 25, pp. 9301-9310 . https://doi.org/10.1021/acssuschemeng.0c00702, ACS Sustainable Chemistry and Engineering, 8 (25, Blossom, B M, Russo, D A, Singh, R K, Van Oort, B, Keller, M B, Simonsen, T, Perzon, A, Gamon, L F, Davies, M J, Cannella, D, Croce, R, Jensen, P E, Bjerrum, M J & Felby, C 2020, ' Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination ', ACS Sustainable Chemistry & Engineering, vol. 8, no. 25, pp. 9301-9310 . https://doi.org/10.1021/acssuschemeng.0c00702, ACS Sustainable Chemistry and Engineering, 8(25), 9301-9310. American Chemical Society, ACS Sustainable Chemistry and Engineering 8 (2020) 25
- Publication Year :
- 2020
-
Abstract
- Photobiocatalysis holds great promise toward the development of sustainable and environmentally friendly processes, harnessing light to drive biocatalytic reactions. However, photobiocatalysis at the interface of insoluble substrates, such as cellulose, has not been studied in much detail. In this context, the catalytic enhancement of lytic polysaccharide monooxygenases (LPMOs) by light is of great interest to the biorefinery field due to their capacity to oxidatively cleave such recalcitrant polysaccharides which can facilitate the degradation of lignocellulose. It has previously been reported that light-driven LPMO reactions have a huge catalytic potential, but effective continuous illumination in reactors may be challenging. Therefore, we investigated the impact of intermittent illumination. We show that illumination intervals as short as 1 s/min enable LPMO catalysis on phosphoric acid-swollen cellulose (PASC) to the same level as continuous illumination. Additionally, time-resolved measurements indicate that reductant depletion, and not enzyme inactivation, limits light-driven LPMO reactions. This study shows that a 60-fold reduction in illumination time enhances LPMO catalysis while protecting reaction elements, e.g. the reductant. Most importantly, the significant enhancement of LPMO catalysis with minimal and intermittent illumination is promising toward an application of photobiocatalytic depolymerization of lignocellulose where shading and light scattering minimize light availability and continuity.<br />SCOPUS: ar.j<br />info:eu-repo/semantics/published
- Subjects :
- Cellulose oxidation
General Chemical Engineering
COPPER
Informatique appliquée logiciel
Photosensitizer
02 engineering and technology
Lytic polysaccharide monooxygenases
Photobiocatalysis
010402 general chemistry
Polysaccharide
OXIDATION
01 natural sciences
chemistry.chemical_compound
Environmental Chemistry
Génie chimique
Chimie
chemistry.chemical_classification
Renewable Energy, Sustainability and the Environment
Chlorophyllin
Reactive oxygen species (ROS)
General Chemistry
Monooxygenase
DEGRADATION
021001 nanoscience & nanotechnology
Environmentally friendly
0104 chemical sciences
Technologie de l'environnement, contrôle de la pollution
Intermittent light
chemistry
Biochemistry
Lytic cycle
CELLULOSE
0210 nano-technology
SDG 6 - Clean Water and Sanitation
ENZYMES
Subjects
Details
- Language :
- English
- ISSN :
- 21680485
- Database :
- OpenAIRE
- Journal :
- ACS Sustainable Chemistry and Engineering, 8(25), 9301-9310, Blossom, B M, Russo, D A, Singh, R K, Van Oort, B, Keller, M B, Simonsen, T I, Perzon, A, Gamon, L F, Davies, M J, Cannella, D, Croce, R, Jensen, P E, Bjerrum, M J & Felby, C 2020, ' Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination ', ACS Sustainable Chemistry and Engineering, vol. 8, no. 25, pp. 9301-9310 . https://doi.org/10.1021/acssuschemeng.0c00702, ACS Sustainable Chemistry and Engineering, 8 (25, Blossom, B M, Russo, D A, Singh, R K, Van Oort, B, Keller, M B, Simonsen, T, Perzon, A, Gamon, L F, Davies, M J, Cannella, D, Croce, R, Jensen, P E, Bjerrum, M J & Felby, C 2020, ' Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination ', ACS Sustainable Chemistry & Engineering, vol. 8, no. 25, pp. 9301-9310 . https://doi.org/10.1021/acssuschemeng.0c00702, ACS Sustainable Chemistry and Engineering, 8(25), 9301-9310. American Chemical Society, ACS Sustainable Chemistry and Engineering 8 (2020) 25
- Accession number :
- edsair.doi.dedup.....fa5e3fe09a9ce33eadc683522a681353