Back to Search
Start Over
Establishment of a mouse model of lipopolysaccharide-induced neutrophilic nasal polyps
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2016
-
Abstract
- Research has identified that gram-negative bacteria have an important role in refractory nasal polyps. In the present study, lipopolysaccharide (LPS) was used to establish a mouse model with neutrophilic nasal polyps in order to explore the effect and mechanism of LPS on the formation of neutrophilic nasal polyps in mice. A total of 5 or 10 µg of LPS was dropped into the nasal cavities of C57BL/6J mice in order to establish animal models with neutrophilic nasal polyps. Histological staining, toll-like receptor 4 (TLR4), cluster of differentiation 68 for macrophages and myeloperoxidase for neutrophil immunohistochemistry were used to observe histopathological changes in the nasal mucosa. The expression levels of cytokines, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-4 and IL-17 in the nasal lavage fluid, were detected by ELISA. Compared with the control group, mice in the LPS groups exhibited significant mucosa epithelial cell damage and nasal polyp formation. Furthermore, TLR4+ cells, macrophages, neutrophils and significantly increased levels of IFN-γ, TNF-α, and IL-17 in the nasal lavage fluids were indicated (all P=0.008). These findings indicated that LPS is able to activate the TLR4 receptor pathway to induce the formation of neutrophilic nasal polyps in mice. Additionally, LPS administration was accompanied by a significant increase in the number of macrophages, T helper (Th) 1 and Th17-related cytokines (P=0.009, P=0.008 and P=0.008, respectively). Therefore, the present model is commensurate with the characteristics of primary nasal polyps that have been identified in the Asian population.
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Lipopolysaccharide
Mucous membrane of nose
Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Immunology and Microbiology (miscellaneous)
neutrophils
medicine
otorhinolaryngologic diseases
Nasal polyps
030223 otorhinolaryngology
nasal polyps
chronic rhinosinusitis
lipopolysaccharide
Interleukin
General Medicine
Articles
medicine.disease
030104 developmental biology
chemistry
Myeloperoxidase
Immunology
TLR4
biology.protein
Tumor necrosis factor alpha
Nasal Lavage Fluid
Subjects
Details
- ISSN :
- 17920981
- Volume :
- 14
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Experimental and therapeutic medicine
- Accession number :
- edsair.doi.dedup.....fa4b2b25376fe4a4f3641faebde389a2