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Streptomyces clavuligerus: The Omics Era
- Source :
- Journal of industrial microbiologybiotechnology. 48(9-10)
- Publication Year :
- 2021
-
Abstract
- The Streptomyces clavuligerus genome consists in a linear chromosome of about 6.7 Mb and four plasmids (pSCL1 to pSCL4), the latter one of 1.8 Mb. Deletion of pSCL4, results in viable mutants with high instability in the chromosome arms, which may lead to chromosome circularisation. Transcriptomic and proteomic studies comparing different mutants with the wild-type strain improved our knowledge on the biosynthesis and regulation of clavulanic acid, cephamycin C and holomycin. Additional knowledge has been obtained on the SARP-type CcaR activator and the network of connections with other regulators (Brp, AreB, AdpA, BldG, RelA) controlling ccaR expression. The transcriptional pattern of the cephamycin and clavulanic acid clusters is supported by the binding of CcaR to different promoters and confirmed that ClaR is a CcaR-dependent activator that controls the late steps of clavulanic biosynthesis. Metabolomic studies allowed the detection of new metabolites produced by S. clavuligerus such as naringenin, desferroxamines, several N-acyl tunicamycins, the terpenes carveol and cuminyl alcohol or bafilomycin J. Heterologous expression of S. clavuligerus terpene synthases resulted in the formation of no less than 15 different terpenes, although none of them was detected in S. clavuligerus culture broth. In summary, application of the Omic tools results in a better understanding of the molecular biology of S. clavuligerus, that allows the use of this strain as an industrial actinobacterial platform and helps to improve CA production.
- Subjects :
- Proteomics
biology
Chemistry
Activator (genetics)
Mutant
Streptomyces clavuligerus
Bioengineering
Promoter
Gene Expression Regulation, Bacterial
biology.organism_classification
Applied Microbiology and Biotechnology
Genome
Streptomyces
Plasmid
Biochemistry
Bacterial Proteins
medicine
Heterologous expression
Cephamycin
Clavulanic Acid
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 14765535
- Volume :
- 48
- Issue :
- 9-10
- Database :
- OpenAIRE
- Journal :
- Journal of industrial microbiologybiotechnology
- Accession number :
- edsair.doi.dedup.....fa493e9671eea38f863056a58bacf94f