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The effect of curcumin on low-passage glioblastoma cells in vitro
- Source :
- Journal of Cancer Research and Therapeutics, Vol 12, Iss 2, Pp 1025-1032 (2016)
- Publication Year :
- 2016
- Publisher :
- Medknow, 2016.
-
Abstract
- Background: Plant extract therapy has been the cornerstone of cancer treatment for many years. The natural component curcumin demonstrated antineoplastic effects on different type of tumor cells. In this study, we explored the effectiveness of curcumin against low-passage human primary glioblastoma (GB) cell cultures. Materials and Methods: Early passage GB cell cultures (GB3B, GB4B, and GB5B) were established from fresh samples tissue obtained from GB patients. Growth rate (GR) and doubling time (DT) was determined for each cell line. The cytotoxic effect of curcumin was quantified by hemocytometer cell counting, using trypan blue. To study the changes in cell shape, GB cells exposed to a concentration corresponding to inhibitory concentration 50 (IC50) of curcumin were studied by phase-contrast microscopy by capturing images during the treatment. Results: Our results showed that GB cells proliferate with a GR of 0.2872 and a DT of 2.41 days for GB3B, a GR of 0.2787 and a DT of 2.49 days for GB4B, and a GR of 0.2787 and a DT of 2.49 days for GB5B. Curcumin induced cell death in GB cells in a time- and dose-dependent manner. The IC50 for GB3B was 46.4 µM, for GB4B was 78,3 µM, and for GB5B was 47.7 µM. Phase contrast microscopy showed that cultures treated with curcumin in a concentration corresponding to IC50 contained rounded cells and cell fragments, 72 h after the treatment. Conclusions: The results of the present investigation proved that curcumin is a natural compound potentially useful in the fight against GB.
- Subjects :
- 0301 basic medicine
Programmed cell death
Curcumin
Cell Survival
Cell
Antineoplastic Agents
lcsh:RC254-282
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Hemocytometer
Cell Line, Tumor
Tumor Cells, Cultured
medicine
Humans
Cytotoxic T cell
Doubling time
Radiology, Nuclear Medicine and imaging
Cell Proliferation
therapy
Dose-Response Relationship, Drug
business.industry
General Medicine
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Molecular biology
030104 developmental biology
medicine.anatomical_structure
Oncology
chemistry
Cell culture
030220 oncology & carcinogenesis
Immunology
Trypan blue
Glioblastoma
business
Subjects
Details
- ISSN :
- 09731482
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer Research and Therapeutics
- Accession number :
- edsair.doi.dedup.....fa21cb5818fdc5c17004decb8348ff40