Back to Search
Start Over
Defective Pollen WallIs Required for Anther and Microspore Development in Rice and Encodes a Fatty Acyl Carrier Protein Reductase
- Source :
- The Plant Cell. 23:2225-2246
- Publication Year :
- 2011
- Publisher :
- Oxford University Press (OUP), 2011.
-
Abstract
- Aliphatic alcohols naturally exist in many organisms as important cellular components; however, their roles in extracellular polymer biosynthesis are poorly defined. We report here the isolation and characterization of a rice (Oryza sativa) male-sterile mutant, defective pollen wall (dpw), which displays defective anther development and degenerated pollen grains with an irregular exine. Chemical analysis revealed that dpw anthers had a dramatic reduction in cutin monomers and an altered composition of cuticular wax, as well as soluble fatty acids and alcohols. Using map-based cloning, we identified the DPW gene, which is expressed in both tapetal cells and microspores during anther development. Biochemical analysis of the recombinant DPW enzyme shows that it is a novel fatty acid reductase that produces 1-hexadecanol and exhibits >270-fold higher specificity for palmiltoyl-acyl carrier protein than for C16:0 CoA substrates. DPW was predominantly targeted to plastids mediated by its N-terminal transit peptide. Moreover, we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 (ms2) mutant and is the probable ortholog of MS2. These data suggest that DPWs participate in a conserved step in primary fatty alcohol synthesis for anther cuticle and pollen sporopollenin biosynthesis in monocots and dicots.
- Subjects :
- Molecular Sequence Data
Mutant
Arabidopsis
Flowers
Plant Science
Cutin
Biology
Pollen exine formation
Microspore
Gene Expression Regulation, Plant
otorhinolaryngologic diseases
Acyl Carrier Protein
Arabidopsis thaliana
Tissue Distribution
Amino Acid Sequence
Plastid
Research Articles
Phylogeny
Plant Proteins
Molecular Structure
Genetic Complementation Test
Gene Expression Regulation, Developmental
food and beverages
Oryza
Cell Biology
Plants, Genetically Modified
biology.organism_classification
Acyl carrier protein
Phenotype
Biochemistry
Mutation
biology.protein
Pollen
Fatty Alcohols
Oxidoreductases
Pollen wall
Subjects
Details
- ISSN :
- 1532298X and 10404651
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- The Plant Cell
- Accession number :
- edsair.doi.dedup.....fa0fda69d15ec561d74a884df6926c91