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Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses
- Source :
- Journal of virological methods. 140(1-2)
- Publication Year :
- 2006
-
Abstract
- This study evaluates the feasibility of the use of the FTA® Gene Guard System (a commercial product consisting of filter paper impregnated with patented chemicals supplied by the Whatman company) for the shipment, storage and detection of RNA rabies viruses by a simplified hemi-nested reverse transcriptase polymerase chain reaction. HnRT-PCR of the rabies virus nucleoprotein gene with specific primers showed that viral RNA extracted from crude infected tissues remained stable after fixation on the filter paper under diverse environmental conditions for at least 35 days. The sequence analysis of the products amplified from five out of the seven known genotypes of Lyssaviruses showed the stability of viral RNA viruses after fixation on the filter paper. Furthermore, the sensitivity of the hnRT-PCR following RNA fixation on the filter paper was equivalent to that of standard hnRT-PCR. In conclusion, the stability of viral RNA and the inactivation of infectivity make the FTA® technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.
- Subjects :
- Paper
Time Factors
Molecular Sequence Data
Foxes
Biology
medicine.disease_cause
Sensitivity and Specificity
Virus
Microbiology
Cell Line
Specimen Handling
Dogs
Virology
Cricetinae
Sequence Homology, Nucleic Acid
medicine
Animals
Cloning, Molecular
Lyssavirus
DNA Primers
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Rabies virus
RNA
Nucleic acid amplification technique
Rhabdoviridae
biology.organism_classification
Reverse transcriptase
Nucleoprotein
Evaluation Studies as Topic
Feasibility Studies
RNA, Viral
Nucleic Acid Amplification Techniques
Filtration
Biotechnology
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 140
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....fa0d578b49c025304f88d2d7e65a360b