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Nitric Oxide as an Autocrine Regulator of Sodium Currents in Baroreceptor Neurons
- Source :
- Neuron. (5):1039-1049
- Publisher :
- Cell Press.
-
Abstract
- Arterial baroreceptors are mechanosensitive nerve endings in the aortic arch and carotid sinus that play a critical role in acute regulation of arterial blood pressure. A previous study has shown that nitric oxide (NO) or NO-related species suppress action potential discharge of baroreceptors. In the present study, we investigated the effects of NO on Na+ currents of isolated baroreceptor neurons in culture. Exogenous NO donors inhibited both tetrodotoxin (TTX) -sensitive and -insensitive Na+ currents. The inhibition was not mediated by cGMP but by NO interaction with channel thiols. Acute inhibition of NO synthase increased the Na+ currents. NO scavengers (hemoglobin and ferrous diethyldithiocarbamate) increased Na+ currents before but not after inhibition of NO synthase. Furthermore, NO production in the neuronal cultures was detected by chemiluminescence and immunoreactivity to the neuronal isoform of NO synthase was identified in fluorescently identified baroreceptor neurons. These results indicate that NO/NO-related species function as autocrine regulators of Na+ currents in baroreceptor neurons. Modulation of Na+ channels may represent a novel response to NO.
- Subjects :
- medicine.medical_specialty
Patch-Clamp Techniques
Baroreceptor
Neuroscience(all)
Pressoreceptors
Tetrodotoxin
Nitric Oxide
Sodium Channels
Nitric oxide
Rats, Sprague-Dawley
Hemoglobins
chemistry.chemical_compound
Internal medicine
medicine
Animals
Cysteine
Ferrous Compounds
Neurons, Afferent
Patch clamp
Autocrine signalling
Cyclic GMP
Aorta
S-Nitrosothiols
Chemistry
General Neuroscience
Sodium
Sulfhydryl Reagents
Carotid sinus
Rats
Autocrine Communication
Hydrazines
Endocrinology
medicine.anatomical_structure
nervous system
Ethylmaleimide
Biophysics
cardiovascular system
Nodose Ganglion
Mechanosensitive channels
Ditiocarb
Ion Channel Gating
Free nerve ending
Nitroso Compounds
Subjects
Details
- Language :
- English
- ISSN :
- 08966273
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....f9fcdab51539b84a5c7e450bc0f73a03
- Full Text :
- https://doi.org/10.1016/S0896-6273(00)80484-5