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Expression of epidermal growth factor variant III (EGFRvIII) in pediatric diffuse intrinsic pontine gliomas
- Publication Year :
- 2012
-
Abstract
- Despite numerous clinical trials over the past 2 decades, the overall survival for children diagnosed with diffuse intrinsic pontine glioma (DIPG) remains 9–10 months. Radiation therapy is the only treatment with proven effect and novel therapies are needed. Epidermal growth factor receptor variant III (EGFRvIII) is the most common variant of the epidermal growth factor receptor and is expressed in many tumor types but is rarely found in normal tissue. A peptide vaccine targeting EGFRvIII is currently undergoing investigation in phase 3 clinical trials for the treatment of newly diagnosed glioblastoma (GBM), the tumor in which this variant receptor was first discovered. In this study, we evaluated EGFRvIII expression in pediatric DIPG samples using immunohistochemistry with a double affinity purified antibody raised against the EGFRvIII peptide. Staining of pediatric DIPG histological samples revealed expression in 4 of 9 cases and the pattern of staining was consistent with what has been seen in EGFRvIII transfected cells as well as GBMs from adult trials. In addition, analysis of tumor samples collected immediately post mortem and of DIPG cells in culture by RT-PCR, western blot analysis, and flow cytometry confirmed EGFRvIII expression. We were therefore able to detect EGFRvIII expression in 6 of 11 DIPG cases. These data suggest that EGFRvIII warrants investigation as a target for these deadly pediatric tumors.
- Subjects :
- Adult
Cancer Research
Pathology
medicine.medical_specialty
medicine.medical_treatment
Blotting, Western
Article
Flow cytometry
Immunoenzyme Techniques
Epidermal growth factor
medicine
Brain Stem Neoplasms
Humans
Epidermal growth factor receptor
RNA, Messenger
In Situ Hybridization, Fluorescence
biology
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
EGFRvIII Peptide
Immunotherapy
Flow Cytometry
Prognosis
Peptide Fragments
Blot
ErbB Receptors
Neurology
Oncology
Child, Preschool
biology.protein
Peptide vaccine
Immunohistochemistry
Neurology (clinical)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....f9f73727be3bf42f9330218832faeae0