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Synergistic anti-hyperalgesia of electroacupuncture and low dose of celecoxib in monoarthritic rats: involvement of the cyclooxygenase activity in the spinal cord
- Source :
- Brain research bulletin. 77(2-3)
- Publication Year :
- 2008
-
Abstract
- Electroacupuncture (EA) can effectively control the exaggerated pain in humans with inflammatory disease and animals with experimental inflammatory pain. However, there have been few investigations on the effect of co-administration of EA and analgesics and the underlying synergistic mechanism. Using behavioral test, RT-PCR analysis, enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA), the present study demonstrated that (1) Unilateral intra-articular injection of complete Freund's adjuvant (CFA) produced a constant hyperalgesia and an up-regulation of the prostaglandin E(2) (PGE(2)) level as well as the tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6 levels in the spinal cord; (2) Celecoxib, a selective inhibitor of cyclooxygenase-2 (COX-2), at a dose of 2, 10, and 20 mg/kg (twice daily, p.o.), presented a dose-dependent anti-hyperalgesic effect; (3) Repeated EA stimulation of ipsilateral 'Huan-Tiao' (GB30) and 'Yang-Ling-Quan' (GB34) acupoints significantly suppressed CFA-induced hyperalgesia, and markedly inhibited the CFA-induced increase of the level of PGE(2) as well as IL-1beta, IL-6, and TNF-alpha in the spinal cord; (4) EA combined with low dose of celecoxib (2 mg/kg, twice daily, p.o.) greatly enhanced the anti-hyperalgesic effects of EA, with a synergistic reversing effect on CFA-induced up-regulation of spinal PGE(2), but not on the IL-1beta, IL-6, or TNF-alpha. These data indicated that repeated EA combined with low dose of celecoxib produced synergistic anti-hyperalgesic effect in the CFA-induced monoarthritic rats, which could be made possible by regulating the activity of spinal COX, hence the spinal PGE(2) level. Thus, this combination may provide an effective strategy for pain management.
- Subjects :
- Male
Hot Temperature
Electroacupuncture
medicine.medical_treatment
Freund's Adjuvant
Interleukin-1beta
Stimulation
Pharmacology
Dinoprostone
Rats, Sprague-Dawley
medicine
Animals
Humans
Cyclooxygenase Inhibitors
Sulfonamides
Lumbar Vertebrae
biology
Behavior, Animal
Dose-Response Relationship, Drug
business.industry
Interleukin-6
Tumor Necrosis Factor-alpha
General Neuroscience
Anti-Inflammatory Agents, Non-Steroidal
Interleukin
Spinal cord
Arthritis, Experimental
Rats
medicine.anatomical_structure
Spinal Cord
Celecoxib
Cyclooxygenase 2
Hyperalgesia
Anesthesia
biology.protein
Pyrazoles
Cyclooxygenase
medicine.symptom
business
medicine.drug
Prostaglandin E
Subjects
Details
- ISSN :
- 18732747
- Volume :
- 77
- Issue :
- 2-3
- Database :
- OpenAIRE
- Journal :
- Brain research bulletin
- Accession number :
- edsair.doi.dedup.....f9f160ab19ca6d24a8dc73acef48f5f7