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Th17/Treg cells regulated by interleukin 6 in the pathogenesis of chronic rhinosinusitis with nasal polyps
- Source :
- European Archives of Oto-Rhino-Laryngology. 279:3493-3501
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- To be investigated whether Th17/Treg cells regulated by Interleukin-6 in the pathogenesis of chronic rhinosinusitis with nasal polyps. The distributions of Th17 and Treg cells in peripheral blood mononuclear cells (PBMCs) and tissues got from 46 CRSwNP patients and 14 controls were evaluated. Th17 and Treg cells and cells-related cytokines in serum were assessed in means of cytometric bead array (CBA) multiplex assays and enzyme-linked immunosorbent assays (ELISAs). Spleen cells were isolated from spleen of 20 normal BALB/c mice (male), isolated and purified with CD4 antibody immunomagnetic bead kit. CD4+ cells were divided into three groups, including TGF-β1, TGF-β1+ IL-6 and control (PBS). Treg and Th17 cells and cells related cytokines were detected by flow cytometry (FCM) after collecting spleen cells. The level of IL‐10 and IL‐17 in supernatant was measured by ELISA. Th17/Treg ratio and the level of IL-6 in both ECRSwNP (P
- Subjects :
- Male
chemical and pharmacologic phenomena
Spleen
T-Lymphocytes, Regulatory
Peripheral blood mononuclear cell
Flow cytometry
Transforming Growth Factor beta1
Pathogenesis
Mice
Nasal Polyps
RAR-related orphan receptor gamma
medicine
Animals
Humans
Nasal polyps
Sinusitis
Interleukin 6
medicine.diagnostic_test
biology
Interleukin-6
business.industry
hemic and immune systems
General Medicine
medicine.disease
medicine.anatomical_structure
Otorhinolaryngology
Chronic Disease
Immunology
Leukocytes, Mononuclear
biology.protein
Cytokines
Th17 Cells
Antibody
business
Subjects
Details
- ISSN :
- 14344726 and 09374477
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- European Archives of Oto-Rhino-Laryngology
- Accession number :
- edsair.doi.dedup.....f9d62b98332cdfce89b42c70559fef31