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Potentiation of immune responses in mice by a new inosine derivative—Methyl inosine monophosphate (MIMP)
- Source :
- International Journal of Immunopharmacology. 14:1259-1266
- Publication Year :
- 1992
- Publisher :
- Elsevier BV, 1992.
-
Abstract
- Inosine 5′ - methyl monophosphate (MIMP) is a new immunomodulator designed to improve upon the activity of other thymomimetic purines. In Balb/c mice, MIMP was assessed for toxicity and activity on immune responses. The lethal dose for half the mice ( LD 50 ) exceeded 500 mg/kg of body weight by both the parenteral and oral routes. At doses of 1 – 100 mg/kg, the mice showed no visible untoward effects. The antibody response of splenocytes to sheep erythrocytes (SRBC) was measured by IgM plaque-forming cells (PFC) in soft agar under optimal conditions of immunization and challenge. MIMP (1 – 100 mg/kg) was given by both the intraperitoneal and oral routes (gavage) at the time of SRBC injection and 4 days thereafter. The PFC response was found to be significantly augmented. The maximum effect (approximately 2 ×) was observed at 50 and 100 mg/kg, via intraperitoneal (i.p.) and oral routes, respectively. Increases (maximally 1.5 ×) in the responses of splenic lymphocytes to mitogen stimulation with phytohemagglutinin (PHA) and concanavalin A (Con A) were observed under similar conditions of MIMP treatment. SRBC-induced delayed-hypersensitivity (DTH) was also measured under optimal conditions. By both i.p. and oral routes, enhancement of DTH response was produced by the lower doses of MIMP (0.01–1 mg/kg). Again, a second peak of optimum stimulation of DTH response was produced by 50 mg/kg of MIMP when administered by both routes. The effect was observed mainly on the sensitization rather than on the expression phase. MIMP qualifies as an effective immunopotentiator in normal mice.
- Subjects :
- medicine.drug_class
T-Lymphocytes
Immunology
chemical and pharmacologic phenomena
Immunopotentiator
Pharmacology
Lymphocyte Activation
Median lethal dose
Immunostimulant
Lethal Dose 50
Mice
Adjuvants, Immunologic
Inosine Monophosphate
Oral administration
medicine
Animals
Hypersensitivity, Delayed
Antibody-Producing Cells
Inosine
Mice, Inbred BALB C
biology
Lethal dose
Concanavalin A
Antibody Formation
Toxicity
biology.protein
Female
medicine.drug
Subjects
Details
- ISSN :
- 01920561
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Journal of Immunopharmacology
- Accession number :
- edsair.doi.dedup.....f9d3e87323bc73c27799cca4169216ca