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Exposure of the major human red-cell glycolipid, globoside, to galactose oxidase
- Source :
- Scopus-Elsevier
-
Abstract
- Plasma membrane glycolipids are localized at the outer leaflet of the lipid bilayer, and their carbohydrate portions are exposed to the environment. The efficiency of exposure has, however, not been known. We have been able to determine the availability of the major red cell glycolipid, globoside, to externally added galactose oxidase. Red cells were extensively treated with the enzyme and the oxidized cells reduced with NaBD4. After isolation the extent of exposed globoside was estimated by mass spectrometry. The results show that the exposure of globoside varies in red cells of different individuals from 37-66%. The fatty acid composition of externally available globoside was the same as that of non-oxidized globoside. The exposure was not influenced by protease treatment of intact cells and no correlation was found with different ABO blood groups.
- Subjects :
- Chemical Phenomena
Borohydrides
Biology
Galactose Oxidase
Biochemistry
Gas Chromatography-Mass Spectrometry
Glycosphingolipids
Glycolipid
medicine
Humans
Lipid bilayer
chemistry.chemical_classification
Globosides
Red Cell
Globoside
Erythrocyte Membrane
Fatty Acids
Carbohydrate
Chemistry
Red blood cell
medicine.anatomical_structure
Enzyme
chemistry
Galactose oxidase
Glycolipids
Oxidation-Reduction
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....f9bc9c9a3a23f83c36192fd93544ef80