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Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1
- Source :
- Journal of Integrative Agriculture, Vol 12, Iss 4, Pp 638-645 (2013)
- Publication Year :
- 2013
- Publisher :
- Elsevier, 2013.
-
Abstract
- Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F 1 plants, F 2 , F 3 and BC 1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F 2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.
- Subjects :
- Agriculture (General)
Population
Puccinia striiformis f. sp. tritici
Psathyrostachys huashanica Keng
Plant Science
Plant disease resistance
Biology
Biochemistry
Genetic analysis
S1-972
Food Animals
Gene mapping
Genetic linkage
education
wheat cultivar
Genetics
education.field_of_study
Ecology
Wheat diseases
resistance genes
Bulked segregant analysis
food and beverages
Genetic marker
Animal Science and Zoology
molecular mapping
Agronomy and Crop Science
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20953119
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....f9a5fe18f4c6d357eca008972b4c8345