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IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS BASED ON PRINCIPLES OF POLYPHASE TAXONOMIC APPROACH
- Source :
- Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 6, Pp 25-34 (2016)
- Publication Year :
- 2016
- Publisher :
- Central Research Institute for Epidemiology, 2016.
-
Abstract
- Aim. Determine an optimal set of the most effective methods of identification and intraspecies typing of causative agents of glanders and melioidosis. Materials and methods. Bacteriologic, immunochemical, molecular-genetic methods were used. Results. A possibility to identify collection strains of pathogenic and closely related Burkholderia in semiautomatic systems is studied. Means of detection of informative variable genome segments of the specified microorganisms were developed, methods of their genetic typing were selected. Effectiveness of application of precipitating mAbs for differentiation of Burkholderia was established. Data on diagnostic possibilities of immunoglobulins fluorescing based on monoclonal antibodies of various etiotropic directionality for detection and identification of B. mallei and B. pseudomallei are generalized. Experimental series of amplification test-systems for identification of glanders and melioidosis causative agents in real-time PCR format are created. Conclusion. A number of methods for identification and typing of glanders and melioidosis causative agents is proposed.
- Subjects :
- 0301 basic medicine
Melioidosis
biology
030106 microbiology
Glanders
Medicine (miscellaneous)
glanders and melioidosis causative agents
General Medicine
Computational biology
biology.organism_classification
medicine.disease
Microbiology
QR1-502
intra-species typing
03 medical and health sciences
Genetic typing
Burkholderia
medicine
Identification (biology)
monoclonal antibodies
Typing
pathogenic burkholderia genomes
Subjects
Details
- ISSN :
- 26867613 and 03729311
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Journal of microbiology, epidemiology and immunobiology
- Accession number :
- edsair.doi.dedup.....f9a38357a5044e7b402768aa8d966abe