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Multispectral quantitative immunohistochemical analysis of tumor-infiltrating lymphocytes in relation to programmed death-ligand 1 expression in triple-negative breast cancer
- Source :
- Breast cancer (Tokyo, Japan). 27(4)
- Publication Year :
- 2020
-
Abstract
- Programmed death-ligand 1 (PD-L1) expression on immune cells (ICs) is a predictive marker for PD-L1 checkpoint blockade in patients with triple-negative breast cancer (TNBC). However, the level of PD-L1 expression and the percentage of cells that are PD-L1+ are continuous variables not dichotomous variables for tumor-infiltrating lymphocytes (TILs) and other cells. Multiplexed immunohistochemistry was applied to 31 archived surgical specimens from untreated TNBC patients. TIL levels were visually scored, and CD8+ T cells and PD-L1+ ICs were quantified using an automated multispectral imaging system. PD-L1 expression was assessed within a multiplexed context (CD8 combined spectral composite). The mean value of stromal TILs (i.e., the percentage of the stromal area with a dese mononuclear infiltrate) was 20%. The frequency of patients with PD-L1-positive tumor cells (TC) and ICs was 38.7% and 32.2%, respectively, with a significant association between them. TIL levels were correlated with CD8+ T cell infiltration in the stroma (Spearman r = 0.795, p
- Subjects :
- 0301 basic medicine
Adult
Stromal cell
Triple Negative Breast Neoplasms
CD8-Positive T-Lymphocytes
B7-H1 Antigen
03 medical and health sciences
0302 clinical medicine
Immune system
Lymphocytes, Tumor-Infiltrating
medicine
Tumor Microenvironment
Humans
Pharmacology (medical)
Radiology, Nuclear Medicine and imaging
Breast
Immune Checkpoint Inhibitors
Triple-negative breast cancer
Aged
Retrospective Studies
Aged, 80 and over
Predictive marker
Tumor-infiltrating lymphocytes
business.industry
General Medicine
Middle Aged
medicine.disease
Immunohistochemistry
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Female
business
Infiltration (medical)
CD8
Subjects
Details
- ISSN :
- 18804233
- Volume :
- 27
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Breast cancer (Tokyo, Japan)
- Accession number :
- edsair.doi.dedup.....f99b08f9c8a434cd878ccb115ed62002