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Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol
- Source :
- Antioxidants, Volume 10, Issue 6, Antioxidants, Vol 10, Iss 959, p 959 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate the optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cultured under hypoxic conditions and the results were compared to the standard ones. We observed a significant increase of CD-56 positive phenotypic marker the ability to form functional myotubes, increase in the proportion of young cells in cell primary suspensions, and a decrease in the percentage of apoptotic cells among PBN-conditioned cells in normoxia an hypoxia. We also observed significantly higher levels of SOD3 expression<br />maintained expression of SOD1, SOD2, and CAT<br />a higher level of BCL2 gene expression<br />and a rather significant decrease in Hsp70 gene expression in PBN-conditioned SkMDS/PCs compared to the WT population under hypoxic conditions. In addition, significant increase of myogenic genes expression was observed after PBN addition to culture medium, compared to WT population under hypoxia. Interestingly, PBN addition significantly increased the lengths of telomeres under hypoxia. Based on the data obtained, we can postulate that PBN conditioning of human SkMDS/PCs could be a promising step in improving myogenic cell preparation protocol for pro-regenerative treatment of post-infarction hearts.
- Subjects :
- 0301 basic medicine
Physiology
Clinical Biochemistry
Population
SOD2
heart failure
RM1-950
medicine.disease_cause
Biochemistry
Article
03 medical and health sciences
0302 clinical medicine
Gene expression
medicine
α-phenyl-N-tert-butyl nitrone
oxidative stress
Progenitor cell
education
Molecular Biology
education.field_of_study
Chemistry
Myogenesis
apoptosis
Cell Biology
Hypoxia (medical)
Cell biology
030104 developmental biology
Apoptosis
post-infarction heart
Therapeutics. Pharmacology
medicine.symptom
skeletal muscle derived stem cells
030217 neurology & neurosurgery
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Antioxidants
- Accession number :
- edsair.doi.dedup.....f98d17f95cae4293693050f087a400f2