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Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study
- Source :
- Cytogenetic and Genome Research, Cytogenetic and Genome Research, Karger, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩, Scopus-Elsevier, Cytogenetics and Cell Genetics, Cytogenetics and Cell Genetics, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩, Cytogenetics and Cell Genetics, S Karger AG, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩
- Publication Year :
- 2001
- Publisher :
- HAL CCSD, 2001.
-
Abstract
- Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.
- Subjects :
- [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
[SDV]Life Sciences [q-bio]
MESH: Cosmids
Mineralogy
Biology
Sensitivity and Specificity
Fluorescence
03 medical and health sciences
Yeasts
Genetics
Humans
MESH: In Situ Hybridization, Fluorescence
DNA, Fungal
Molecular Biology
Image resolution
Chromosomes, Artificial, Yeast
Genetics (clinical)
Image restoration
In Situ Hybridization, Fluorescence
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
MESH: Chromosomes, Artificial, Yeast
MESH: Humans
Fragment (computer graphics)
business.industry
MESH: Yeasts
030302 biochemistry & molecular biology
Resolution (electron density)
MESH: Fluorescence
Pattern recognition
Fibroblasts
Cosmids
MESH: Sensitivity and Specificity
MESH: DNA, Fungal
MESH: Fibroblasts
MESH: DNA Probes
Fish
Deconvolution
Artificial intelligence
business
DNA Probes
Subjects
Details
- Language :
- English
- ISSN :
- 14248581, 1424859X, 03010171, and 14219816
- Database :
- OpenAIRE
- Journal :
- Cytogenetic and Genome Research, Cytogenetic and Genome Research, Karger, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩, Scopus-Elsevier, Cytogenetics and Cell Genetics, Cytogenetics and Cell Genetics, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩, Cytogenetics and Cell Genetics, S Karger AG, 2001, 92 (1-2), pp.59-62. ⟨10.1159/000056869⟩
- Accession number :
- edsair.doi.dedup.....f98cdc936efecdab1ce5a79c9dc87f52
- Full Text :
- https://doi.org/10.1159/000056869⟩