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La/SSB ribonucleoprotein levels increased in transformed cells
- Source :
- Clinical and Experimental Immunology. 83:369-374
- Publication Year :
- 1991
- Publisher :
- Oxford University Press (OUP), 1991.
-
Abstract
- SUMMARY Autoantibodies to the La/SSB ribonucleoprotein are commonly found in patients with Sjögren's syndrome. Previous studies have shown that La/SSB accumulates in cells shortly after viral infection. We have extended these studies by investigating levels of the La/SSB antigen in virally and spontaneously transformed cell lines (contact-insensitive and tumourigenic) relative to their non-transformed counterpart cell lines (contact-sensitive and non-tumourigenic). Transformed BALB/ 3T12-3 and KNRK fibroblasts were visibly brighter by immunofluorcscence assay than non-transformed BALB/3T3 and NRK fibroblasts respectively, when reacted with anti-La/SSB specific sera. This was confirmed by flow cytometry, as La/SSB levels were elevated in the transformed counterparts of the same cell lines. An anti-Sm monoclonal antibody and normal human serum reacted with these cell lines failed to show a significant increase by flow cytometry. Finally, a two-fold increase in the La/SSB antigen was demonstrated in cell lysates of these cell lines by a capture ELISA. These data show that La/SSB is elevated in transformed cell lines compared with non-transformed counterpart cell lines and suggest that this increase is not restricted to viral transformation.
- Subjects :
- medicine.drug_class
Immunology
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Viral transformation
Biology
Monoclonal antibody
Immunofluorescence
Autoantigens
Flow cytometry
Sarcoma Viruses, Murine
Mice
stomatognathic system
Antigen
medicine
Animals
Immunology and Allergy
Fibroblast
Cell Line, Transformed
Ribonucleoprotein
medicine.diagnostic_test
Fibroblasts
Cell Transformation, Viral
Flow Cytometry
Rats
stomatognathic diseases
medicine.anatomical_structure
Ribonucleoproteins
Cell culture
Research Article
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....f98c7235de5ad96de92e3da3d23facb7