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High Concentrations of Polyelectrolyte Complex Nanoparticles Decrease Activity of Osteoclasts
- Source :
- Molecules, Volume 24, Issue 12, Molecules, Vol 24, Iss 12, p 2346 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Fracture treatment in osteoporotic patients is still challenging. Osteoporosis emerges when there is an imbalance between bone formation and resorption in favor of resorption by osteoclasts. Thus, new implant materials for osteoporotic fracture treatment should promote bone formation and reduce bone resorption. Nanoparticles can serve as drug delivery systems for growth factors like Brain-Derived Neurotrophic Factor (BDNF), which stimulated osteoblast differentiation. Therefore, polyelectrolyte complex nanoparticles (PEC-NPs) consisting of poly(l-lysine) (PLL) and cellulose sulfate (CS), with or without addition of BDNF, were used to analyze their effect on osteoclasts in vitro. Live cell images showed that osteoclast numbers decreased after application of high PLL/CS PEC-NPs concentrations independent of whether BDNF was added or not. Real-time RT-PCR revealed that relative mRNA expression of cathepsin K and calcitonin receptor significantly declined after incubation of osteoclasts with high concentrations of PLL/CS PEC-NPs. Furthermore, Enzyme-Linked Immunosorbent Assay indicated that tartrate-resistant acidic phosphatase 5b activity was significantly reduced in the presence of high PLL/CS PEC-NPs concentrations. Consistent with these results, the pit formation analysis showed that less hydroxyapatite was resorbed by osteoclasts after incubation with high concentrations of PLL/CS PEC-NPs. BDNF had no influence on osteoclasts. We conclude that highly concentrated PLL/CS PEC-NPs dosages decreased osteoclastogenesis and osteoclasts activity. Moreover, BDNF might be a promising growth factor for osteoporotic fracture treatment since it did not increase osteoclast activity.
- Subjects :
- musculoskeletal diseases
medicine.medical_specialty
medicine.medical_treatment
Osteoporosis
Gene Expression
Pharmaceutical Science
cathepsin K
Article
Bone resorption
Analytical Chemistry
lcsh:QD241-441
polyelectrolyte complex nanoparticles
03 medical and health sciences
0302 clinical medicine
lcsh:Organic chemistry
Osteogenesis
Osteoclast
Internal medicine
Drug Discovery
calcitonin receptor
Cathepsin K
medicine
Humans
drug delivery system
Physical and Theoretical Chemistry
Calcitonin receptor
030304 developmental biology
0303 health sciences
Chemistry
Growth factor
Organic Chemistry
Cell Differentiation
Osteoblast
medicine.disease
Polyelectrolytes
Resorption
osteoclasts
BDNF
medicine.anatomical_structure
Endocrinology
pit formation
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Nanoparticles
Molecular Medicine
tartrate-resistant acidic phosphatase 5b
Biomarkers
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....f98c42dca19276d5cf60acb36c765c65