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Identification and potential functions of tRNA-derived small RNAs (tsRNAs) in irritable bowel syndrome with diarrhea
- Source :
- Pharmacological Research. 173:105881
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- IBS-D is a functional bowel disease without clear diagnostic markers and exact pathogenesis. Studies have proved that non-coding RNAs participate in IBS-D. However, tRNA-derived small RNAs (tsRNAs), as a new type of non-coding RNAs that are more suitable as markers, remain to be clarified in IBS-D. Hence, we focused on the identification and potential functions of tsRNAs in IBS-D. Intestinal biopsies were obtained from IBS-D patients and healthy volunteers, and twenty-eight differential tsRNAs were screened by high-throughput sequencing. The changes of tiRNA-His-GTG-001, tRF-Ser-GCT-113, and tRF-Gln-TTG-035 by q-PCR in expanded samples were consistent with the sequencing results. Meanwhile, target gene prediction and bioinformatics showed that the three differential tsRNAs may be involved in some key signal pathways, such as GABAergic synapse, tumor necrosis factor-α (TNF-α), etc. Their regulation on target genes were demonstrated through cell experiments and luciferase reporter assays. In addition, the receiver-operating characteristic (ROC) analysis showed that the three tsRNAs all could be used as candidate markers of IBS-D. The correlation analysis indicated they were related to the degree of abdominal pain, abdominal distension, and stool morphology. So, we believe that the abnormal tiRNA-His-GTG-001, tRF-Ser-GCT-113, and tRF-Gln-TTG-035 are related to the clinical symptoms of IBS-D, and can target regulate the important molecules of the brain-gut axis, even could be expected as potential biomarkers for the diagnosis and treatment of IBS-D.
- Subjects :
- Adult
Diarrhea
Male
Abdominal pain
Biology
Bioinformatics
Cell Line
Irritable Bowel Syndrome
Pathogenesis
Young Adult
RNA, Transfer
medicine
Humans
Intestinal Mucosa
Gene
Irritable bowel syndrome
Pharmacology
High-Throughput Nucleotide Sequencing
Middle Aged
Abdominal distension
medicine.disease
Biomarker (cell)
Female
Tumor necrosis factor alpha
medicine.symptom
Biomarkers
Subjects
Details
- ISSN :
- 10436618
- Volume :
- 173
- Database :
- OpenAIRE
- Journal :
- Pharmacological Research
- Accession number :
- edsair.doi.dedup.....f9765061c03cf3f24d69e5c71a4a8bd8