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Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffolds
- Source :
- Tissue engineering. Part C, Methods. 21(2)
- Publication Year :
- 2014
-
Abstract
- To date, expansion of bone-marrow-derived mesenchymal stem cells (MSCs) is typically carried out on two-dimensional (2D) tissue culture plastic. Since this 2D substratum is very different from the physiological situation, MSCs gradually lose their unique multipotent properties during expansion. Recently, the role of the extracellular matrix (ECM) microenvironment (“niche”) in facilitating and regulating stem cell behavior in vivo has been elucidated. As a result, investigators have shifted their efforts toward developing three-dimensional (3D) scaffolds capable of functioning like the native tissue ECM. In this study, we demonstrated that stromal-cell-derived ECM, formed within a collagen/hydroxyapatite (Col/HA) scaffold to mimic the bone marrow “niche,” promoted MSC proliferation and preserved their differentiation capacity. The ECM was synthesized by MSCs to reconstitute the tissue-specific 3D microenvironment in vitro. Following deposition of the ECM inside Col/HA scaffold, the construct was decellularized and reseeded with MSCs to study their behavior. The data showed that MSCs cultured on the ECM-Col/HA scaffolds grew significantly faster than the cells from the same batch cultured on the regular Col/HA scaffolds. In addition, MSCs cultured on the ECM-Col/HA scaffolds retained their “stemness” and osteogenic differentiation capacity better than MSCs cultured on regular Col/HA scaffolds. When ECM-Col/HA scaffolds were implanted into immunocompromised mice, with or without loading MSCs, it was found that those scaffolds formed less bone as compared with regular Col/HA scaffolds (i.e., without ECM), in both cases of with or without loading MSCs. The in vivo study further confirmed that the ECM-Col/HA scaffold was a suitable mimic of the bone marrow “niche.” This novel 3D stromal-cell-derived ECM system has the potential to be developed into a biomedical platform for regenerative medicine applications.
- Subjects :
- Adult
Scaffold
Stromal cell
Cellular differentiation
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
Mice, SCID
Article
Extracellular matrix
Colony-Forming Units Assay
Tissue culture
Young Adult
Implants, Experimental
Osteogenesis
medicine
Animals
Humans
RNA, Messenger
Cells, Cultured
Cell Proliferation
Tissue Scaffolds
Chemistry
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
X-Ray Microtomography
Immunohistochemistry
Cell biology
Extracellular Matrix
Kinetics
medicine.anatomical_structure
Cattle
Bone marrow
Stem cell
Stromal Cells
Biomarkers
Biomedical engineering
Subjects
Details
- ISSN :
- 19373392
- Volume :
- 21
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Tissue engineering. Part C, Methods
- Accession number :
- edsair.doi.dedup.....f961114caa6bfdbfd461283b1966badb