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Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (44), pp.16562-7. ⟨10.1073/pnas.0603511103⟩, Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (44), pp.16562-7. ⟨10.1073/pnas.0603511103⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- Synaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs spontaneously at low intraterminal calcium concentrations and is stimulated by a rise in intracellular calcium. Exocytosis is compensated for by the reformation of vesicles at plasma membrane and endosomes. Although the adaptor complex AP-3 was proposed to be involved in the formation of SVs from endosomes, whether its function has an indirect effect on exocytosis remains unknown. Using mocha mice, which are deficient in functional AP-3, we identify an AP-3-dependent tetanus neurotoxin-resistant asynchronous release that can be evoked at hippocampal mossy fiber (MF) synapses. Presynaptic targeting of the tetanus neurotoxin-resistant vesicle soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) is lost in mocha hippocampal MF terminals, whereas the localization of synaptobrevin 2 is unaffected. In addition, quantal release in mocha cultures is more frequent and more sensitive to sucrose. We conclude that lack of AP-3 results in more constitutive secretion and loss of an asynchronous evoked release component, suggesting an important function of AP-3 in regulating SV exocytosis at MF terminals.
- Subjects :
- Quantitative Biology - Subcellular Processes
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Hippocampal formation
Synaptic Transmission
Calcium in biology
MESH: Synapses
Tissue Culture Techniques
Mice
chemistry.chemical_compound
0302 clinical medicine
MESH: Animals
Neurotransmitter
Hippocampal mossy fiber
0303 health sciences
Multidisciplinary
MESH: Electrophysiology
Chemistry
MESH: Transcription Factors
Biological Sciences
Cell biology
DNA-Binding Proteins
Electrophysiology
MESH: Calcium
Mossy Fibers, Hippocampal
Neurons and Cognition (q-bio.NC)
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Adaptor Protein Complex 3
Endosome
MESH: Mice, Transgenic
Mice, Transgenic
Neurotransmission
Sensitivity and Specificity
Synaptic vesicle
Exocytosis
03 medical and health sciences
Osmotic Pressure
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
MESH: Synaptic Transmission
Animals
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
MESH: Tissue Culture Techniques
Subcellular Processes (q-bio.SC)
MESH: Mice
030304 developmental biology
Brefeldin A
MESH: Brefeldin A
MESH: Mossy Fibers, Hippocampal
MESH: Osmotic Pressure
MESH: Sensitivity and Specificity
FOS: Biological sciences
Quantitative Biology - Neurons and Cognition
Synapses
Calcium
Neuroscience
030217 neurology & neurosurgery
MESH: DNA-Binding Proteins
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (44), pp.16562-7. ⟨10.1073/pnas.0603511103⟩, Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (44), pp.16562-7. ⟨10.1073/pnas.0603511103⟩
- Accession number :
- edsair.doi.dedup.....f944f77f5c78d908560da1306f63f8ed