Back to Search
Start Over
Zn Subcellular Distribution in Liver of Goldfish (Carassius Auratus) with Exposure to Zinc Oxide Nanoparticles and Mechanism of Hepatic Detoxification
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 11, p e78123 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- Zinc Oxide Nanoparticles (ZnO NPs) have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut) of goldfish (Carassius auratus) after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+). And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L), bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+), the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut), rather than transport to the internal tissues (liver and muscle). The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG) increased significantly, and after Zn(2+) exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.
- Subjects :
- inorganic chemicals
Gills
Cations, Divalent
lcsh:Medicine
Nanoparticle
chemistry.chemical_element
Zinc
medicine.disease_cause
Divalent
Toxicology
Goldfish
medicine
Metallothionein
Animals
Intestinal Mucosa
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
Chemistry
Muscles
lcsh:R
Granule (cell biology)
technology, industry, and agriculture
Intestines
Liver
Bioaccumulation
Zinc toxicity
Toxicity
Inactivation, Metabolic
Biophysics
Nanoparticles
lcsh:Q
Zinc Oxide
Water Pollutants, Chemical
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....f9413408a99a6da4b1a55603df577c7b