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Establishment of Photosynthesis through Chloroplast Development Is Controlled by Two Distinct Regulatory Phases
- Source :
- Plant Physiology, 176, 1199
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Chloroplasts develop from undifferentiated proplastids present in meristematic tissue. Thus, chloroplast biogenesis is closely connected to leaf development, which restricts our ability to study the process of chloroplast biogenesis per se. As a consequence, we know relatively little about the regulatory mechanisms behind the establishment of the photosynthetic reactions and how the activities of the two genomes involved are coordinated during chloroplast development. We developed a single cell-based experimental system from Arabidopsis (Arabidopsis thaliana) with high temporal resolution allowing for investigations of the transition from proplastids to functional chloroplasts. Using this unique cell line, we could show that the establishment of photosynthesis is dependent on a regulatory mechanism involving two distinct phases. The first phase is triggered by rapid light-induced changes in gene expression and the metabolome. The second phase is dependent on the activation of the chloroplast and generates massive changes in the nuclear gene expression required for the transition to photosynthetically functional chloroplasts. The second phase also is associated with a spatial transition of the chloroplasts from clusters around the nucleus to the final position at the cell cortex. Thus, the establishment of photosynthesis is a two-phase process with a clear checkpoint associated with the second regulatory phase allowing coordination of the activities of the nuclear and plastid genomes.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Nuclear gene
Physiology
Research Articles - Focus Issue
Arabidopsis
Light-Harvesting Protein Complexes
Plant Science
Photosynthesis
Zea mays
01 natural sciences
Genome
Cell Line
03 medical and health sciences
chloroplast
Gene Expression Regulation, Plant
Plant Cells
Botany
Genetics
Arabidopsis thaliana
Plastids
Plastid
Feedback, Physiological
photosynthesis
biology
Arabidopsis Proteins
ta1183
food and beverages
Cell Differentiation
biology.organism_classification
Cell biology
Plant Leaves
Chloroplast
030104 developmental biology
light
signaling
Biogenesis
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15322548, 00320889, and 09218971
- Volume :
- 176
- Database :
- OpenAIRE
- Journal :
- Plant Physiology
- Accession number :
- edsair.doi.dedup.....f9346a3cf5c192d351ef0ec14390ee7e