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The structure of SSO2064, the first representative of Pfam family PF01796, reveals a novel two-domain zinc-ribbon OB-fold architecture with a potential acyl-CoA-binding role
- Source :
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Acta crystallographica. Section F, Structural biology and crystallization communications, vol 66, iss Pt 10
- Publication Year :
- 2010
- Publisher :
- International Union of Crystallography (IUCr), 2010.
-
Abstract
- The crystal structure of SSO2064, the first structural representative of Pfam family PF01796 (DUF35), reveals a two-domain architecture comprising an N-terminal zinc-ribbon domain and a C-terminal OB-fold domain. Analysis of the domain architecture, operon organization and bacterial orthologs combined with the structural features of SSO2064 suggests a role involving acyl-CoA binding for this family of proteins.<br />SSO2064 is the first structural representative of PF01796 (DUF35), a large prokaryotic family with a wide phylogenetic distribution. The structure reveals a novel two-domain architecture comprising an N-terminal, rubredoxin-like, zinc ribbon and a C-terminal, oligonucleotide/oligosaccharide-binding (OB) fold domain. Additional N-terminal helical segments may be involved in protein–protein interactions. Domain architectures, genomic context analysis and functional evidence from certain bacterial representatives of this family suggest that these proteins form a novel fatty-acid-binding component that is involved in the biosynthesis of lipids and polyketide antibiotics and that they possibly function as acyl-CoA-binding proteins. This structure has led to a re-evaluation of the DUF35 family, which has now been split into two entries in the latest Pfam release (v.24.0).
- Subjects :
- Models, Molecular
Protein Folding
Domains of Unknown Function
acyl-coA
Plasma protein binding
Crystallography, X-Ray
Biochemistry
Protein structure
Models
Genome, Archaeal
Structural Biology
Acyl-CoA-binding protein
2.1 Biological and endogenous factors
Aetiology
Peptide sequence
0303 health sciences
Crystallography
Genome
030302 biochemistry & molecular biology
Biological Sciences
Condensed Matter Physics
Zinc
Sulfolobus solfataricus
polyketide biosynthesis
Protein folding
Biotechnology
Protein Binding
Protein Structure
1.1 Normal biological development and functioning
Archaeal Proteins
Molecular Sequence Data
Biophysics
Biology
acyl-carrier proteins
Structural genomics
03 medical and health sciences
Polyketide
Underpinning research
Genetics
Amino Acid Sequence
030304 developmental biology
Oligonucleotide
Molecular
structural genomics
Protein Structure, Tertiary
Archaeal
Chemical Sciences
X-Ray
Acyl Coenzyme A
Tertiary
Subjects
Details
- ISSN :
- 17443091
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Accession number :
- edsair.doi.dedup.....f91c27720e0f8f92586076dbfcb67b4c