Back to Search
Start Over
S-Palmitoylation Sorts Membrane Cargo for Anterograde Transport in the Golgi
- Source :
- Developmental Cell, Developmental Cell, Elsevier, 2018, 47 (4), pp.479-493.e7. ⟨10.1016/j.devcel.2018.10.024⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; While retrograde cargo selection in the Golgi is known to depend on specific signals, it is unknown whether anterograde cargo is sorted, and anterograde signals have not been identified. We suggest here that S-palmitoylation of anterograde cargo at the Golgi membrane interface is an anterograde signal and that it results in concentration in curved regions at the Golgi rims by simple physical chemistry. The rate of transport across the Golgi of two S-palmitoylated membrane proteins is controlled by S-palmitoylation. The bulk of S-palmitoylated proteins in the Golgi behave analogously, as revealed by click chemistry-based fluorescence and electron microscopy. These palmitoylated cargos concentrate in the most highly curved regions of the Golgi membranes, including the fenestrated perimeters of cisternae and associated vesicles. A palmitoylated transmembrane domain behaves similarly in model systems.
- Subjects :
- 0301 basic medicine
Lipoylation
Golgi Apparatus
macromolecular substances
Biology
Endoplasmic Reticulum
environment and public health
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Palmitoylation
Golgi
Humans
Protein palmitoylation
Molecular Biology
Cells, Cultured
030304 developmental biology
Golgi membrane
0303 health sciences
Membranes
Trafficking
[PHYS.PHYS]Physics [physics]/Physics [physics]
Chemistry
Vesicle
Sorting
technology, industry, and agriculture
Biological Transport
Intracellular Membranes
Cell Biology
Golgi apparatus
Cell biology
Transport protein
Transmembrane domain
Protein Transport
030104 developmental biology
Membrane
S-palmitoylation
Membrane protein
Axoplasmic transport
Biophysics
Posttranslational modification
symbols
lipids (amino acids, peptides, and proteins)
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15345807
- Database :
- OpenAIRE
- Journal :
- Developmental Cell, Developmental Cell, Elsevier, 2018, 47 (4), pp.479-493.e7. ⟨10.1016/j.devcel.2018.10.024⟩
- Accession number :
- edsair.doi.dedup.....f918ecc034218ecce55d1492ea0bf1f5