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Wnt3a promotes melanin synthesis of mouse hair follicle melanocytes
- Source :
- Biochemical and Biophysical Research Communications. 420:799-804
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Although the importance of Wnt3a in melanocyte development has been well recognized, the effect of Wnt3a in normal HF melanocytes has not been clearly elucidated yet. Thus, we sought to examine the presence and location of Wnt3a in HF during hair cycle. By using melanocyte-targeted Dct-LacZ transgenic mice, we found that Wnt3a signaling is activated in mouse HF melanocytes during anagen of hair cycle. To further explore the potential functions of Wnt3a in mouse melanocytes, we infected melan-a cells with AdWnt3a to serve as the production source of Wnt3a protein. We demonstrated that Wnt3a promoted melanogenesis through upregulation of MITF and its downstream genes, tyrosinase and TRP1, in melanocytes. In vivo, AdWnt3a rescued the effects of AdsimMITF on HF melanocytes and promoted melanin synthesis. Our results suggest that Wnt3a plays an important role in mouse HF melanocytes homeostasis.
- Subjects :
- Genetically modified mouse
animal structures
Tyrosinase
Biophysics
Mice, Transgenic
Melanocyte
Biology
Biochemistry
Cell Line
Wnt3 Protein
Mice
Downregulation and upregulation
Hair cycle
medicine
Animals
Molecular Biology
Melanins
integumentary system
Stem Cells
Cell Biology
beta-Galactosidase
Microphthalmia-associated transcription factor
Hair follicle
body regions
medicine.anatomical_structure
embryonic structures
Cancer research
Melanocytes
Hair Follicle
WNT3A
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 420
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....f8d33834c8f7b49ddd107a4c8693278d