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Changes in protein synthesis on mitomycin C induction of wild-type and mutant CloDF13 plasmids
- Source :
- Journal of bacteriology. 130(2)
- Publication Year :
- 1977
-
Abstract
- Mitomycin C treatment of Escherichia coli K-12 cells containing the nonconjugative plasmid CloDF13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two CloDF13-specified proteins whose molecular weights correspond to cloacin and immunity protein. Five molecules of immunity protein were synthesized for each cloacin DF13 molecule. Mitomycin C-treated cells containing a copy mutant of CloDF13 made three to four times as much of each protein as cells containing wild-type CloDF13. CloDF13 plasmids that contained the transposon Tn1 were isolated. Two did not induce after mitomycin C treatment, failing both to inhibit host cell synthesis and to produce the two new proteins. In minicells, they showed reduced CloDF13-specified protein synthesis and produced three Tn1-specified proteins.
- Subjects :
- Mitomycin
Mutant
Colicins
Biology
medicine.disease_cause
Cloacin
Microbiology
Mitomycins
Plasmid
Bacteriocins
Host chromosome
Klebsiella
Protein biosynthesis
medicine
Escherichia coli
Molecular Biology
Mitomycin C
Wild type
Molecular biology
Molecular Weight
Kinetics
Colicin
Mutation
DNA Transposable Elements
Plasmids
Research Article
Subjects
Details
- ISSN :
- 00219193
- Volume :
- 130
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of bacteriology
- Accession number :
- edsair.doi.dedup.....f8d15fc486599f31849791fc8c9e63c8