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The nanoscale spatial organization of B-cell receptors on immunoglobulin M- and G-expressing human B-cells
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2016
-
Abstract
- In resting and activated B-cells, IgM and IgG B-cell receptors (BCRs) reside in highly heterogeneous protein islands that vary in size and number of BCRs but intrinsically maintain a high frequency of BCR monomers. IgG BCRs are more clustered than IgM BCRs on resting cells and form larger protein islands after antigen activation.<br />B-cell activation is initiated by the binding of antigen to the B-cell receptor (BCR). Here we used dSTORM superresolution imaging to characterize the nanoscale spatial organization of immunoglobulin M (IgM) and IgG BCRs on the surfaces of resting and antigen-­activated human peripheral blood B-cells. We provide insights into both the fundamental process of antigen-driven BCR clustering and differences in the spatial organization of IgM and IgG BCRs that may contribute to the characteristic differences in the responses of naive and memory B-cells to antigen. We provide evidence that although both IgM and IgG BCRs reside in highly heterogeneous protein islands that vary in size and number of BCR single-molecule localizations, both resting and activated B-cells intrinsically maintain a high ­frequency of single isolated BCR localizations, which likely represent BCR monomers. IgG BCRs are more clustered than IgM BCRs on resting cells and form larger protein islands after antigen activation. Small, dense BCR clusters likely formed via protein–protein interactions are present on the surface of resting cells, and antigen activation induces these to come together to form less dense, larger islands, a process likely governed, at least in part, by protein–lipid interactions.
- Subjects :
- 0301 basic medicine
B-cell receptor
Receptors, Antigen, B-Cell
Lymphocyte Activation
03 medical and health sciences
Spatio-Temporal Analysis
Antigen
Humans
Antigens
Receptor
Molecular Biology
Spatial organization
B-Lymphocytes
biology
Cell Membrane
breakpoint cluster region
Cell Biology
Articles
Superresolution
Molecular biology
Peripheral blood
Cell biology
030104 developmental biology
Immunoglobulin M
Cell Biology of Disease
Immunoglobulin G
biology.protein
Signal Transduction
Subjects
Details
- ISSN :
- 19394586
- Volume :
- 28
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular biology of the cell
- Accession number :
- edsair.doi.dedup.....f8cfb9b792e1b7ec4117482fec384022