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Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
- Source :
- Sensors, Volume 21, Issue 5, Sensors, Vol 21, Iss 1681, p 1681 (2021), Sensors (Basel, Switzerland)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transcription Polymerase Chain Reaction (RT-PCR) real time analysis that requires specialized equipment, reagents and facilities and typically 3–4 h to perform. Thus, the realization of simple, low-cost, small-size, rapid and point-of-care diagnostics tests has become a global priority. In response to the current need for quick, highly sensitive and on-site detection of the SARS-CoV-2 virus in several aqueous solutions, a specific molecularly imprinted polymer (MIP) receptor has been designed, realized, and combined with an optical sensor. More specifically, the proof of concept of a SARS-CoV-2 sensor has been demonstrated by exploiting a plasmonic plastic optical fiber sensor coupled with a novel kind of synthetic MIP nano-layer, especially designed for the specific recognition of Subunit 1 of the SARS-CoV-2 Spike protein. First, we have tested the effectiveness of the developed MIP receptor to bind the Subunit 1 of the SARS-CoV-2 spike protein, then the results of preliminary tests on SARS-CoV-2 virions, performed on samples of nasopharyngeal (NP) swabs in universal transport medium (UTM) and physiological solution (0.9% NaCl), were compared with those obtained with RT-PCR. According to these preliminary results, the sensitivity of the proposed optical-chemical sensor proved to be higher than the RT-PCR one. Furthermore, a relatively fast response time (about 10 min) to the virus was obtained without the use of additional reagents.
- Subjects :
- Optical fiber
Materials science
Protein subunit
viruses
02 engineering and technology
lcsh:Chemical technology
01 natural sciences
Biochemistry
Sensitivity and Specificity
Virus
Article
Analytical Chemistry
law.invention
Molecularly Imprinted Polymers
law
Humans
lcsh:TP1-1185
Electrical and Electronic Engineering
Plastic optical fiber
Instrumentation
Plasmon
Optical Fibers
Optical fiber sensor
SARS-CoV-2
optical fiber sensors
Optical-chemical sensor
optical-chemical sensors
010401 analytical chemistry
Molecularly imprinted polymer
Response time
COVID-19
021001 nanoscience & nanotechnology
Molecularly Imprinted Polymer
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Proof of concept
Spike Glycoprotein, Coronavirus
molecularly imprinted polymers (MIPs)
0210 nano-technology
Biological system
surface plasmon resonance (SPR)
Spike Glycoprotein, Coronaviru
Human
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....f8c9fc569de1e630fd2853b98fef8b33
- Full Text :
- https://doi.org/10.3390/s21051681