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Effects of murine viral leukemia on spleen nucleoside deaminase: Purification and properties of the enzyme from leukemic spleen

Effects of murine viral leukemia on spleen nucleoside deaminase: Purification and properties of the enzyme from leukemic spleen

Authors :
Robert Silber
V.G. Malathi
Source :
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 238:377-387
Publication Year :
1971
Publisher :
Elsevier BV, 1971.

Abstract

A 400-fold increase was observed in the specific activity of nucleoside deaminase (cytidine aminohydrolase, EC 3.5.4.5) in the spleen of mice infected with Friend murine leukemia virus. The enzyme was purified 100-fold from the spleen of leukemic mice. It was sensitive to SH reagents and stimulated by dithiothreitol and GSH. Different pH responses were observed in the presence or absence of dithiothreitol. Cytidine was deaminated at a greater rate than deoxycytidine; cytosine or its nucleotides were not deaminated. The molecular weight determined by gel filtration was 74 000.

Details

ISSN :
00052787
Volume :
238
Database :
OpenAIRE
Journal :
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
Accession number :
edsair.doi.dedup.....f8c5bebf51f991a4faa8bd1a1a5e04b7
Full Text :
https://doi.org/10.1016/0005-2787(71)90612-5