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BNIP3 expression in bovine follicle and corpus luteum
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2018
- Publisher :
- Japanese Society of Veterinary Science, 2018.
-
Abstract
- BNIP3 (BCL2/adenovirus E1B nineteen kilodalton interacting protein-3), a member of the BCL2 family, is activated under hypoxic conditions and induces apoptosis or mitochondrial autophagy for adapting cells to hypoxia. The physiological roles of BNIP3 in the mammalian ovary are still unclear. In order to understand the role of BNIP3 in the bovine ovary, we examined its mRNA and protein expressions of BNIP3 in follicular granulosa cells and corpus luteum (CL). BNIP3 mRNA and protein expressions in granulosa cells from large follicles (>10 mm) at the follicular stage were much higher than those in small follicles (2–8 mm). BNIP3 mRNA and protein expressions in the CL peaked at the early luteal stage. In bovine granulosa cells cultured for 6 hr under hypoxia (3% O2) and normoxia (20% O2), BNIP3 mRNA expression was higher under hypoxia. These results of the present study suggest that BNIP3 has some roles in luteal formation in the bovine ovary, and that the highly expressed BNIP3 in the granulosa cells from large follicles at the follicular stage is related to the roles of BNIP3 in the luteal formation.
- Subjects :
- luteal cell
0301 basic medicine
endocrine system
Granulosa cell
Gene Expression
Estrous Cycle
030209 endocrinology & metabolism
Luteal phase
Andrology
03 medical and health sciences
Follicle
0302 clinical medicine
Corpus Luteum
Proto-Oncogene Proteins
Follicular phase
medicine
Animals
RNA, Messenger
Cells, Cultured
Estrous cycle
Messenger RNA
Granulosa Cells
Full Paper
General Veterinary
hypoxia
urogenital system
Chemistry
bovine
granulosa cell
Cell Hypoxia
030104 developmental biology
medicine.anatomical_structure
Apoptosis
Cattle
Female
ovary
Theriogenology
Corpus luteum
Subjects
Details
- ISSN :
- 13477439 and 09167250
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Medical Science
- Accession number :
- edsair.doi.dedup.....f8c2df58011df170db39c659c22060f2