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Molecular Methods for the Epidemiological Typing of Salmonella enterica Serotype Typhi from Hong Kong and Vietnam
- Source :
- Journal of Clinical Microbiology. 38:292-300
- Publication Year :
- 2000
- Publisher :
- American Society for Microbiology, 2000.
-
Abstract
- A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with Pst I, and total DNA fingerprinting with Nar I. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi.
- Subjects :
- Microbiology (medical)
Serotype
Time Factors
Microbial Sensitivity Tests
Salmonella typhi
DNA, Ribosomal
Typhoid fever
Microbiology
Ribotyping
medicine
Typing
Serotyping
Typhoid Fever
Deoxyribonucleases, Type II Site-Specific
Bacteriophage Typing
Phage typing
Molecular Epidemiology
Molecular epidemiology
biology
Reproducibility of Results
Bacteriology
Drug Resistance, Microbial
biology.organism_classification
medicine.disease
DNA Fingerprinting
Virology
Bacterial Typing Techniques
Vietnam
Salmonella enterica
Hong Kong
Plasmids
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....f8ba468be9115e3ba2c89fb505f592be