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Carcinogenic potential of tobacco tar-resistant Staphylococcus aureus in buccal cavity
- Source :
- Journal of cancer research and clinical oncology. 130(5)
- Publication Year :
- 2004
-
Abstract
- The effects of cigarette smoking on the association between inflammation and cancer were studied, since some bacteria induce the production of tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine and endogenous tumor promoter, in cells.Bacteria from a gargled solution from the buccal cavity of 20 individuals were cultured in the presence of 4 mg/ml cigarette-smoke condensates. Although cigarette-smoke condensates inhibited growth of Staphylococcus aureus strongly and that of Staphylococcus warneri weakly, tobacco tar-resistant S. aureus and S. warneri were obtained.One tobacco tar-resistant S. aureus strain (Sa-TA10) induced expression of the TNF-alpha gene in both Bhas 42 cells (v-Ha-ras transfected BALB/3T3 cells) and human lung cancer cell line H226B, while one tobacco tar-resistant S. warneri (Sw-TA75) did not induce it significantly. Moreover, Sa-TA10 induced formation of transformed foci and soft-agar colony in Bhas 42 cells in cooperation with the v-Ha-ras gene. The results suggested that Sa-TA10 has carcinogenic potential, whereas Sw-TA75 does not.These data suggest that tobacco tar-resistant S. aureus, with carcinogenic potential, is present in the buccal cavity of some individuals, and that cigarette smoking simultaneously inhibits growth of most of the bacteria and selects carcinogenic bacteria.
- Subjects :
- Adult
Cancer Research
Staphylococcus aureus
Lung Neoplasms
medicine.medical_treatment
Gene Expression
medicine.disease_cause
Proinflammatory cytokine
Microbiology
Mice
Tar (tobacco residue)
Drug Resistance, Bacterial
Medicine
Animals
Humans
RNA, Messenger
Carcinogen
Cells, Cultured
Mice, Inbred BALB C
biology
business.industry
Tumor Necrosis Factor-alpha
Mouth Mucosa
General Medicine
3T3 Cells
biology.organism_classification
Cytokine
Cell Transformation, Neoplastic
Oncology
Staphylococcus warneri
Immunology
Carcinogens
Tumor necrosis factor alpha
Tobacco Smoke Pollution
business
Carcinogenesis
Subjects
Details
- ISSN :
- 01715216
- Volume :
- 130
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of cancer research and clinical oncology
- Accession number :
- edsair.doi.dedup.....f894cca553fc1037293af91418e80ac2