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Screening of serum biomarkers and establishment of a decision tree in silica-exposed populations by surface-enhanced laser desorption ionization time-of-fly mass spectrometry
- Source :
- Journal of occupational and environmental medicine. 49(7)
- Publication Year :
- 2007
-
Abstract
- Objective: To screen for serum biomarkers of silicosis and to study their roles using surface-enhanced laser desorption ionization time-of-fly mass spectrometry (SELDI-TOF-MS) techniques. Methods: The serum protein/ peptide profile on the CM10 proteinchip was acquired using SELDI-TOF-MS from workers classified by the Chinese national diagnostic standard for pneumoconiosis. Discriminant analysis was performed to establish a decision tree using protein/peptide peaks. Results: Protein/ peptide peaks changed much more in silica-exposed populations than they did in the control. Discriminant analysis using Wilks' lambda method could give high sensitivity and specificity in distinguishing the silica-exposed population from the control; the decision tree could give 100% sensitivity and 95 % specificity in distinguishing the silicosis phase among the control group. Conclusions: Special proteins/peptides may change in silica-exposed workers and these changes may be used to distinguish silica-exposed populations from the control with the proper discriminant analytical method.
- Subjects :
- Male
medicine.medical_specialty
China
Population
Silicosis
Decision tree
Peptide
Mass spectrometry
medicine
Humans
Mass Screening
education
Mass screening
chemistry.chemical_classification
education.field_of_study
Chromatography
Chemistry
Pneumoconiosis
Decision Trees
Public Health, Environmental and Occupational Health
Discriminant Analysis
Linear discriminant analysis
medicine.disease
Surgery
Surface-enhanced laser desorption/ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Peptides
Biomarkers
Subjects
Details
- ISSN :
- 10762752
- Volume :
- 49
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of occupational and environmental medicine
- Accession number :
- edsair.doi.dedup.....f88cc1b884cefc7e29e1d143f537e0be