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Protection of mice against pandemic H1N1 influenza virus challenge after immunization with baculovirus-expressed stabilizing peptide fusion hemagglutinin protein
- Source :
- Journal of microbiology and biotechnology. 25(2)
- Publication Year :
- 2014
-
Abstract
- Current influenza vaccines are produced in embryonated chicken eggs. However, egg-based vaccines have various problems. To address these problems, recombinant protein vaccines have been developed as new vaccine candidates. Unfortunately, recombinant proteins frequently encounter aggregation and low stability during their biogenesis. It has been previously demonstrated that recombinantly expressed proteins can be greatly stabilized with high solubility by fusing stabilizing peptide (SP) derived from the C-terminal acidic tail of human synuclein (ATS). To investigate whether SP fusion proteins can induce protective immunity in mice, we produced influenza HA and SP fusion protein using a baculovirus expression system. In in vitro tests, SP-fused recombinant HA1 (SP-rHA1) was shown to be more stable than recombinant HA1 (rHA1). Mice were immunized intramuscularly with baculovirus-expressed rHA1 protein or SP-rHA1 protein (2 μg/mouse) formulated with aluminum hydroxide. Antibody responses were determined by ELISA and hemagglutination inhibition assay. We observed that SP-rHA1 immunization elicited HA-specific antibody responses that were comparable to rHA1 immunization. These results indicate that fusion of SP to rHA1 does not negatively affect the immunogenicity of the vaccine candidate. Therefore, it is possible to apply SP fusion technology to develop stable recombinant protein vaccines with high solubility.
- Subjects :
- Recombinant Fusion Proteins
Synucleins
Hemagglutinin Glycoproteins, Influenza Virus
medicine.disease_cause
Antibodies, Viral
Applied Microbiology and Biotechnology
Virus
law.invention
Mice
Influenza A Virus, H1N1 Subtype
Orthomyxoviridae Infections
law
Influenza A virus
medicine
Animals
Humans
Mice, Inbred BALB C
Hemagglutination assay
biology
Immunogenicity
Vaccination
General Medicine
Virology
Fusion protein
Antibodies, Neutralizing
Immunity, Humoral
Disease Models, Animal
Influenza Vaccines
Vaccines, Subunit
Recombinant DNA
biology.protein
Antibody
Baculoviridae
Biotechnology
Subjects
Details
- ISSN :
- 17388872
- Volume :
- 25
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of microbiology and biotechnology
- Accession number :
- edsair.doi.dedup.....f887af45469c669d62d24590414b2e0d