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Phosphatidylinositol 4-Kinaseβ Is Critical for Functional Association of rab11 with the Golgi Complex
- Source :
- Molecular Biology of the Cell. 15:2038-2047
- Publication Year :
- 2004
- Publisher :
- American Society for Cell Biology (ASCB), 2004.
-
Abstract
- Phosphatidylinositol 4-kinasebeta (PI4Kbeta) plays an essential role in maintaining the structural integrity of the Golgi complex. In a search for PI4Kbeta-interacting proteins, we found that PI4Kbeta specifically interacts with the GTP-bound form of the small GTPase rab11. The PI4Kbeta-rab11 interaction is of functional significance because inhibition of rab11 binding to PI4Kbeta abolished the localization of rab11 to the Golgi complex and significantly inhibited transport of vesicular stomatitis virus G protein from the Golgi complex to the plasma membrane. We propose that a novel function of PI4Kbeta is to act as a docking protein for rab11 in the Golgi complex, which is important for biosynthetic membrane transport from the Golgi complex to the plasma membrane.
- Subjects :
- DNA, Complementary
Endosome
Green Fluorescent Proteins
Golgi Apparatus
Saccharomyces cerevisiae
Biology
Transfection
Cell membrane
symbols.namesake
chemistry.chemical_compound
Viral Envelope Proteins
Cricetinae
Two-Hybrid System Techniques
medicine
Animals
Phosphatidylinositol
Molecular Biology
Secretory pathway
Glutathione Transferase
Binding Sites
Brefeldin A
Membrane Glycoproteins
Cell Membrane
Biological Transport
DNA
Articles
Cell Biology
COPI
Golgi apparatus
beta-Galactosidase
Protein Structure, Tertiary
Cell biology
Luminescent Proteins
Phosphotransferases (Alcohol Group Acceptor)
medicine.anatomical_structure
Microscopy, Fluorescence
chemistry
Biochemistry
rab GTP-Binding Proteins
COS Cells
symbols
Guanosine Triphosphate
Protein Binding
PI4KB
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....f8695aa8ac485dddd7fd8bad15e01bee