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No impact of vitamin D on the CYP3A biomarker 4β-hydroxycholesterol in patients with abnormal glucose regulation
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 4, p e0121984 (2015)
- Publication Year :
- 2014
-
Abstract
- Purpose To investigate the effect of vitamin D3 on hepatic Cytochrome P450 enzyme (CYP) 3A4 in patients with abnormal glucose regulation using the endogenous marker 4β-hydroxycholesterol (4β-OHC):cholesterol ratio. Methods The present study took advantage of a trial primarily aiming to investigate the effect of vitamin D3 on beta cell function and insulin sensitivity in patients with abnormal glucose regulation. 44 subjects were randomized to receive vitamin D3, 30000 IU given orally once weekly or placebo for 8 weeks. The two sample t-test was used to test the means of the intra-individual differences of 4β-OHC:cholesterol ratio between the two groups. Results Mean (SD) 4β-OHC in the whole group of patients before and after the intervention was 26 (11) ng/ml and 26 (12). Mean (SD) 4β-OHC:cholesterol ratio in the whole group of patients before and after the intervention was 0.12 (0.046) and 0.13 (0.047). In the Vitamin D group mean (SD) serum 25-OH-vitamin D3 increased from 46 (16) to 85nM (13) during the corresponding time period. To investigate the impact of vitamin D3 on hepatic CYP3A4 we calculated the mean intra-individual differences in 4β-OHC:cholesterol ratio (delta 4β-OHC:cholesterol ratio) before versus after the intervention in the two treatment groups. The difference (95% CI) between delta 4β-OHC:cholesterol ratio in the control group and intervention group was -0.0010 (-0.0093, 0.0072), a difference being not statistically significant (p = 0.80). Conclusions We provide further evidence that vitamin D3 may not substantially affect hepatic CYP3A4. This does not exclude the possibility of an impact of intestinal first-pass metabolism of orally administered drugs which should be investigated. Trial Registration ClinicalTrials.gov NCT01497132
- Subjects :
- Vitamin
Blood Glucose
Male
medicine.medical_specialty
medicine.medical_treatment
lcsh:Medicine
Gene Expression
Biology
Placebo
Body Mass Index
chemistry.chemical_compound
Insulin resistance
Double-Blind Method
Internal medicine
Diabetes mellitus
medicine
Vitamin D and neurology
Diabetes Mellitus
Cytochrome P-450 CYP3A
Humans
Vitamin D
lcsh:Science
Aged
Glucose tolerance test
Multidisciplinary
medicine.diagnostic_test
Cholesterol
Insulin
lcsh:R
Fasting
Glucose Tolerance Test
Middle Aged
medicine.disease
Hydroxycholesterols
Endocrinology
chemistry
Liver
lcsh:Q
Female
Insulin Resistance
Biomarkers
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....f8628509639d6607ef26beffccbc3a24