Back to Search
Start Over
Oxygen evolution in the thylakoid-lacking cyanobacterium Gloeobacter violaceus PCC 7421
- Source :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1777:369-378
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- The oxygen-evolving reactions of the thylakoid-lacking cyanobacterium Gloeobacter violaceus PCC 7421 were compared with those of Synechocystis sp. PCC 6803. Four aspects were considered: sequence conservation in three extrinsic proteins for oxygen evolution, steady-state oxygen-evolving activity, charge recombination reactions, i.e., thermoluminescence and oscillation patterns of delayed luminescence on a second time scale and delayed fluorescence on the nanosecond time scale at − 196 °C. Even though there were significant differences between the amino acid sequences of extrinsic proteins in G. violaceus and Synechocystis sp. PCC 6803, the oxygen-evolving activities were similar. The delayed luminescence oscillation patterns and glow curves of thermoluminescence were essentially identical between the two species, and the nanosecond delayed fluorescence spectral profiles and lifetimes were also very similar. These results indicate clearly that even though the oxygen-evolving reactions are carried out in the periplasm by components with altered amino acid sequences, the essential reaction processes for water oxidation are highly conserved. In contrast, we observed significant changes on the reduction side of photosystem II. Based on these data, we discuss the oxygen-evolving activity of G. violaceus .
- Subjects :
- Cyanobacteria
Luminescence
Photosystem II
Molecular Sequence Data
Biophysics
Cytochrome c Group
Biology
Photochemistry
Biochemistry
chemistry.chemical_compound
Bacterial Proteins
Amino Acid Sequence
Delayed luminescence
chemistry.chemical_classification
Manganese
Synechocystis
Oxygen evolution
Photosystem II Protein Complex
DCMU
Cell Biology
Periplasmic space
biology.organism_classification
Amino acid
Oxygen
Spectrometry, Fluorescence
chemistry
Thylakoid
Extrinsic protein
Sequence Alignment
Gloeobacter violaceus PCC 7421
Recombination
Subjects
Details
- ISSN :
- 00052728
- Volume :
- 1777
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Accession number :
- edsair.doi.dedup.....f851eba520bb5587d2f6bf3d4d420220