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Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation
- Source :
- Catalysts, Volume 10, Issue 8, Catalysts, Vol 10, Iss 851, p 851 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills.
- Subjects :
- Anoxybacillus
010501 environmental sciences
lcsh:Chemical technology
01 natural sciences
Geobacillus
biodegradation
Catalysis
lcsh:Chemistry
03 medical and health sciences
Bioremediation
PAHs
alkane hydroxylase
lipase
lcsh:TP1-1185
Food science
Physical and Theoretical Chemistry
Lipase
crude oil
030304 developmental biology
0105 earth and related environmental sciences
Alkane
chemistry.chemical_classification
0303 health sciences
biology
Chemistry
Thermophile
Biodegradation
lcsh:QD1-999
biology.protein
Organosulfur compounds
thermophiles
Subjects
Details
- Language :
- English
- ISSN :
- 20734344
- Database :
- OpenAIRE
- Journal :
- Catalysts
- Accession number :
- edsair.doi.dedup.....f83c9c72eecc74be6fe68e6d388eb47d
- Full Text :
- https://doi.org/10.3390/catal10080851