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Alanylglutamine Relieved Asthma Symptoms by Regulating Gut Microbiota and the Derived Metabolites in Mice
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2020 (2020), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2020
- Publisher :
- Hindawi, 2020.
-
Abstract
- Objective. Allergic asthma is a chronic inflammatory disease, which seriously affects the life quality of patients, especially children. Alanylglutamine is a nutritional supplement with potential protective and anti-inflammatory effects, but its function in allergic asthma remains elusive. In this study, we focused on the investigations of the roles and functional mechanism of Alanylglutamine in asthma. Methods. Ovalbumin (OVA) induction was utilized to establish a mouse asthma model. 16S rDNA sequencing was performed to compare the diversity of intestinal microorganisms under different treatments. Gas chromatography was utilized to screen the intestinal microbe-short-chain fatty acids in the stool. The lung tissue was extracted to determine signaling pathways, including AMPK, NF-κB, mTOR, STAT3, IKKβ, TGF-β, and IL-1β through Western blot or RT-qPCR. Results. It was observed that Alanylglutamine reduced the cytokine in OVA-induced allergic asthma mice. H&E staining showed obvious pneumonia symptoms in the asthma group, while Alanylglutamine alleviated the inflammatory infiltration. Alanylglutamine reversed gut microbiota compositions in OVA-induced allergic asthma mice and enhanced the butyric acid level. The protective role of Alanylglutamine may be associated with the gut microbiota-butyric acid-GPR43 pathway in asthma mice. In contrast to the OVA group, Alanylglutamine activated the protein expression of P-AMPK/AMPK and inhibited the protein expression of P-mTOR/mTOR, P-P65/P65, P-STAT3/STAT3, P-IKKβ/IKKβ, TGF-β, and IL-1β, with similar effects from butyric acid. Conclusion. The results indicated that Alanylglutamine might be beneficial for asthma, and its effect was achieved through the regulation on microbiota and the derived metabolites. The therapeutic effects might be associated with AMPK, NF-κB, mTOR, and STAT3 signaling pathways. These findings will help identify effective therapeutic direction to alleviate allergic inflammation of the lungs and airways.
- Subjects :
- Male
STAT3 Transcription Factor
0301 basic medicine
Aging
Article Subject
Ovalbumin
medicine.medical_treatment
Gut flora
Biochemistry
Allergic inflammation
Feces
03 medical and health sciences
0302 clinical medicine
Hypersensitivity
medicine
Animals
Amino Acids
PI3K/AKT/mTOR pathway
Asthma
Inflammation
Mice, Inbred BALB C
QH573-671
biology
business.industry
NF-kappa B
AMPK
Biodiversity
Dipeptides
Cell Biology
General Medicine
medicine.disease
biology.organism_classification
Anti-Bacterial Agents
Gastrointestinal Microbiome
030104 developmental biology
Cytokine
030220 oncology & carcinogenesis
Immunology
Metabolome
biology.protein
Butyric Acid
Cytokines
Alanylglutamine
Cytology
business
Signal Transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19420900
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....f82f62ae75c01df20646b073aa5bc94c
- Full Text :
- https://doi.org/10.1155/2020/7101407