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The cyl Genes Reveal the Biosynthetic and Evolutionary Origins of the Group B Streptococcus Hemolytic Lipid, Granadaene
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Vol 10 (2020), Frontiers in Microbiology, Vol 11 (2020), Digibug. Repositorio Institucional de la Universidad de Granada, instname
- Publication Year :
- 2020
- Publisher :
- Frontiers Media SA, 2020.
-
Abstract
- Group B Streptococcus (GBS) is a b-hemolytic, Gram-positive bacterium that commonly colonizes the female lower genital tract and is associated with fetal injury, preterm birth, spontaneous abortion, and neonatal infections. A major factor promoting GBS virulence is the b-hemolysin/cytolysin, which is cytotoxic to several host cells. We recently showed that the ornithine rhamnolipid pigment, Granadaene, produced by the gene products of the cyl operon, is hemolytic. Here, we demonstrate that heterologous expression of the GBS cyl operon conferred hemolysis, pigmentation, and cytoxicity to Lactococcus lactis, a model non-hemolytic Gram-positive bacterium. Similarly, pigment purified from L. lactis is hemolytic, cytolytic, and identical in structure to Granadaene extracted from GBS, indicating the cyl operon is sufficient for Granadaene production in a heterologous host. Using a systematic survey of phyletic patterns and contextual associations of the cyl genes, we identify homologs of the cyl operon in physiologically diverse Gram-positive bacteria and propose undescribed functions of cyl gene products. Together, these findings bring greater understanding to the biosynthesis and evolutionary foundations of a key GBS virulence factor and suggest that such potentially toxic lipids may be encoded by other bacteria.<br />This work was supported by funding from the National Institutes of Health grants R01AI112619, R01AI33976, R01AI00989, and R21AI125907 to LR. The NIH training grant T32AI07509 (PI: Lee Ann Campbell) supported CW. LI and LA were supported by intramural funds from NLM, NIH.
- Subjects :
- Group B Streptococcus
Microbiology (medical)
Operon
lcsh:QR1-502
Heterologous
Virulence
virulence factor
Microbiology
lcsh:Microbiology
Virulence factor
microbial evolution
03 medical and health sciences
bacterial toxin
Gene
Original Research
030304 developmental biology
0303 health sciences
biology
Microbial evolution
030306 microbiology
Lactococcus lactis
Correction
Bacterial toxin
Gram-positive bacteria
biology.organism_classification
3. Good health
bacteria
Heterologous expression
Cytolysin
Subjects
Details
- ISSN :
- 1664302X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....f82c87371962e1686d67840ceb836b54