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Synthesis, characterization, antimicrobial and cytotoxicity evaluation of quaternary cadmium (II)-quercetin complexes with 1,10-phenanthroline or 2,2’-bipyridine ligands
- Source :
- Biotechnology & Biotechnological Equipment, Vol 34, Iss 1, Pp 999-1012 (2020)
- Publication Year :
- 2020
- Publisher :
- Taylor & Francis, 2020.
-
Abstract
- This study reports the synthesis and antibacterial evaluation of two novel complexes, [Cd (Q) (Bpy) (CH3COO)2, complex 1] and [Cd (Q) (Phen) (CH3COO)2, complex 2], based on quercetin ligand. The method of synthesis was by reacting natural flavonoid quercetin (Q) with a good chelator (Bpy = 2,2’-bipyridine, Phen= 1,10-phenanthroline and Q = quercetin) and metal ions. The produced metal complexes were studied in the solid state by Fourier-transform infrared (FTIR) spectroscopy and in solution by UV-Vis absorption. Further analysis included high resolution mass spectrometry (HRMS) for confirmation. To understand the nature and coordination of quercetin and its metal complexes, density functional theory (DFT) calculation was performed. The scavenging (DPPH radical), antibacterial, MTT, enzymatic and non-enzymatic antioxidant activity assay, cytotoxicity assay (fluorescence study) were done and quercetin was used for comparison. Both complex 1 and complex 2 induced loss of cell viability via impairment of metabolic activity, leakage of intracellular proteins, and increased oxidative stress. The free-radical scavenging activity of complex 2 (IC50 340.175 µg/mL) was statistically significantly more potent than that of complex 1. The MIC values of complex 2 (7.80 µg/mL Escherichia coli, 15.62 µg/mL Staphylococcus aureus) were higher as compared to complex 1 and quercetin in both test microorganisms. There was inhibition of cell proliferation in Escherichia coli treated with 2 µg/mL of complex 2, whereas Staphylococcus aureus did not show inhibition at this concentration. The cytotoxicity screening on MG 63 cell line showed that the compounds were safe up to 500 mg/L.
- Subjects :
- 0106 biological sciences
heteroleptic complexes
lcsh:Biotechnology
Phenanthroline
chemistry.chemical_element
01 natural sciences
Medicinal chemistry
2,2'-Bipyridine
quercetin
03 medical and health sciences
chemistry.chemical_compound
free-radical scavenging activity
lcsh:TP248.13-248.65
scavenging activity
Chelation
Cytotoxicity
030304 developmental biology
Cadmium acetate
0303 health sciences
Cadmium
Ligand
enzymatic and non-enzymatic
cadmium acetate
chelates
chemistry
Quercetin
010606 plant biology & botany
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Biotechnology & Biotechnological Equipment, Vol 34, Iss 1, Pp 999-1012 (2020)
- Accession number :
- edsair.doi.dedup.....f812ee1d366ea354533f25ea3d94e802
- Full Text :
- https://doi.org/10.6084/m9.figshare.12930886.v2