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Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering
- Publication Year :
- 2016
- Publisher :
- SPIE, 2016.
-
Abstract
- The application of x-ray phase contrast computed tomography (PCT) to the field of tissue engineering is dis- cussed. Specific focus is on the edge illumination PCT method, which can be adapted to weakly coherent x-ray sources, permitting PCT imaging in standard (non-synchrotron) laboratory environments. The method was applied to a prominent research topic in tissue engineering, namely the development of effective and reliable decellularization protocols to derive scaffolds from native tissue. Results show that edge illumination PCT provides sufficient image quality to evaluate the microstructural integrity of scaffolds and, thus, to assess the performance of the used decellularization technique. In order to highlight that edge illumination PCT can ultimately comply with demands on a high specimen throughput and low doses of radiation, recently developed strategies for scan time and dose reduction are discussed.
- Subjects :
- 0301 basic medicine
Phase contrast tomography
Scaffold
Decellularization
Materials science
Image quality
business.industry
Phase contrast microscopy
Low dose
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA)
law.invention
03 medical and health sciences
030104 developmental biology
Optics
Tissue engineering
law
Tomography
business
low-dose, phase contrast tomography, microstructural scaffolds
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....f809cb14b81a83d3ce949382b816e70e