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Epibrassinolide induces changes in indole-3-acetic acid, abscisic acid and polyamine concentrations and enhances antioxidant potential of radish seedlings under copper stress
- Source :
- Physiologia plantarum. 140(3)
- Publication Year :
- 2010
-
Abstract
- In the present study, the effects of epibrassinolide (EBL) on indole-3-acetic acid (IAA), abscisic acid (ABA) and polyamine (PA) tissue concentrations and antioxidant potential of 7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress were investigated. EBL treatment alone or in combination with Cu enhanced free and bound IAA titers when compared with the metal alone. Modest increases in free and bound ABA contents were observed for EBL treatment alone. However, the combination of EBL with Cu caused major increases in both forms of ABA, over Cu alone. Among the PAs analyzed, only putrescine and cadaverine concentrations were enhanced by EBL treatment alone. By contrast, a significant decline in putrescine and spermine contents was found in seedlings treated with EBL plus Cu. EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) and protein contents in comparison with metal and control treatments. A major decrease in malondialdehyde content was also recorded for EBL treatments with or without Cu. An increase in phytochelatin content was also observed in seedlings treated with EBL alone or in combination with Cu. Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings.
- Subjects :
- Antioxidant
PLANT ABIOTIC STRESS
Physiology
medicine.medical_treatment
Spermine
BRASSINOLIDES
Plant Science
Antioxidants
Raphanus
chemistry.chemical_compound
Steroids, Heterocyclic
Brassinosteroids
Genetics
medicine
Polyamines
Abscisic acid
Cadaverine
Indoleacetic Acids
PLANT HORMONES
RADISH
Cell Biology
General Medicine
Ascorbic acid
Oxidative Stress
chemistry
Biochemistry
Seedlings
Putrescine
Lipid Peroxidation
Indole-3-acetic acid
Polyamine
Cholestanols
Copper
Abscisic Acid
Subjects
Details
- ISSN :
- 13993054
- Volume :
- 140
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Physiologia plantarum
- Accession number :
- edsair.doi.dedup.....f7ff298697275f8b18d2c056aeb26a63