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Methionine restriction slows down senescence in human diploid fibroblasts

Authors :
Christoph Ruckenstuhl
Frank Madeo
Michael Neuhaus
Rafal Koziel
Rudolf J. Wiesner
Maria Bust
Christine Netzberger
Pidder Jansen-Dürr
Eva Albertini
Source :
Aging Cell
Publication Year :
2014
Publisher :
Wiley, 2014.

Abstract

Methionine restriction (MetR) extends lifespan in animal models including rodents. Using human diploid fibroblasts (HDF), we report here that MetR significantly extends their replicative lifespan, thereby postponing cellular senescence. MetR significantly decreased activity of mitochondrial complex IV and diminished the accumulation of reactive oxygen species. Lifespan extension was accompanied by a significant decrease in the levels of subunits of mitochondrial complex IV, but also complex I, which was due to a decreased translation rate of several mtDNA-encoded subunits. Together, these findings indicate that MetR slows down aging in human cells by modulating mitochondrial protein synthesis and respiratory chain assembly.

Details

ISSN :
14749726 and 14749718
Volume :
13
Database :
OpenAIRE
Journal :
Aging Cell
Accession number :
edsair.doi.dedup.....f7f237748a42c465ed4fbd235e0272c1
Full Text :
https://doi.org/10.1111/acel.12266